Takihata Yasuhiro, Kawauchi Satoko, Ogata Sho, Nishidate Izumi, Sato Shunichi, Yamamoto Junji, Kishi Yoji
Department of Surgery, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama 359-8513, Japan.
Division of Bioinformation and Therapeutic Systems, National Defense Medical College Research Institute, 3-2 Namiki, Tokorozawa, Saitama 359-8513, Japan.
Surg Open Sci. 2021 Jul 24;6:21-28. doi: 10.1016/j.sopen.2021.07.002. eCollection 2021 Oct.
Nonalcoholic steatohepatitis is a progressive liver disease that can lead to cirrhosis, hepatocellular carcinoma, and hepatic failure. Thus, the diagnosis of nonalcoholic steatohepatitis, especially discrimination from nonalcoholic fatty liver, is crucial, but reliable methods other than invasive biopsy have not been established yet. In this study, we investigated the usefulness of diffuse reflectance spectroscopy, which does not require tissue collection, to evaluate the pathological states of fatty liver with inflammation.
We performed in vivo optical fiber-based diffuse reflectance spectroscopy in both the near-infrared and visible spectral regions for livers in STAM mice, which typically show steatosis at 6 weeks, steatohepatitis at 8 weeks, and fibrosis at 12 weeks of age. After diffuse reflectance spectroscopy, all of the liver tissues were histologically analyzed and scored on the basis of the rodent nonalcoholic fatty liver disease scoring system. We examined correlations between the diffuse reflectance spectra and scores associated with steatosis and inflammation.
The results showed that the second derivative values of reflectance at 1204 nm, the lipid absorption peak in the near-infrared region, were strongly correlated with steatosis scores (r = 0.9172, P < .0001, n = 20) and that the differences of the first derivative values of reflectance in the visible region (570 nm - 550 nm) that reflect hemoglobin deoxygenation were significantly correlated with inflammation scores (r = 0.5260, P = .0172, n = 20). These results suggest that our diffuse reflectance spectroscopy method is useful for diagnosis of the states of steatosis with inflammation in livers and hence nonalcoholic steatohepatitis.
非酒精性脂肪性肝炎是一种进行性肝病，可导致肝硬化、肝细胞癌和肝衰竭。因此，非酒精性脂肪性肝炎的诊断，尤其是与非酒精性脂肪肝的鉴别诊断至关重要，但除了侵入性活检外，尚未建立可靠的诊断方法。在本研究中，我们研究了无需组织采集的漫反射光谱法在评估伴有炎症的脂肪肝病理状态方面的实用性。
我们对STAM小鼠的肝脏进行了基于光纤的体内漫反射光谱研究，该小鼠在6周龄时通常出现脂肪变性，8周龄时出现脂肪性肝炎，12周龄时出现肝纤维化。在漫反射光谱检查后，对所有肝脏组织进行组织学分析，并根据啮齿动物非酒精性脂肪性肝病评分系统进行评分。我们研究了漫反射光谱与脂肪变性和炎症相关评分之间的相关性。
结果显示，近红外区域脂质吸收峰1204nm处反射率的二阶导数值与脂肪变性评分密切相关（r = 0.9172，P <.0001，n = 20），而反映血红蛋白脱氧的可见光区域（570nm - 550nm）反射率的一阶导数值差异与炎症评分显著相关（r = 0.5260，P =.0172，n = 20）。这些结果表明，我们的漫反射光谱法可用于诊断肝脏脂肪变性伴炎症状态，从而诊断非酒精性脂肪性肝炎。
