Lee Yunjung, Jee Hee-Jung, Noh Hyungjoon, Kang Geun-Hyung, Park Juyeun, Cho Janggeun, Cho Jee-Hyun, Ahn Sangdoo, Lee Chulhyun, Kim Ok-Hee, Oh Byung-Chul, Kim Hyeonjin
Lee Gil Ya Cancer and Diabetes Institute, Gachon University, Incheon, Korea.
Magn Reson Med. 2013 Sep;70(3):620-9. doi: 10.1002/mrm.24510. Epub 2012 Sep 28.
The applicability of the in vivo proton magnetic resonance spectroscopy hepatic lipid profiling (MR-HLP) technique in nonalcoholic fatty liver disease was investigated. Using magnetic resonance spectroscopy, the relative fractions of diunsaturated (fdi), monounsaturated (fmono), and saturated (fsat) fatty acids as well as total hepatic lipid content were estimated in the livers of 8 control and 23 CCl4-treated rats at 9.4 T. The mean steatosis, necrosis, inflammation, and fibrosis scores of the treated group were all significantly higher than those of the control group (P < 0.01). There was a strong correlation between the histopathologic parameters and the MR-HLP parameters (r = 0.775, P < 0.01) where both steatosis and fibrosis are positively correlated with fmono and negatively correlated with fdi. Both necrosis and inflammation, however, were not correlated with any of the MR-HLP parameters. Hepatic lipid composition appears to be changed in association with the severity of steatosis and fibrosis in nonalcoholic fatty liver disease, and these changes can be depicted in vivo by using the MR-HLP method at 9.4 T. Thus, while it may not likely be that MR-HLP helps differentiate between steatohepatitis in its early stages and simple steatosis, these findings altogether are in support of potential applicability of in vivo MR-HLP at high field in nonalcoholic fatty liver disease.
研究了体内质子磁共振波谱肝脏脂质谱分析（MR-HLP）技术在非酒精性脂肪性肝病中的适用性。使用磁共振波谱，在9.4 T场强下对8只对照大鼠和23只四氯化碳处理大鼠的肝脏中双不饱和脂肪酸（fdi）、单不饱和脂肪酸（fmono）和饱和脂肪酸（fsat）的相对含量以及肝脏总脂质含量进行了估计。处理组的平均脂肪变性、坏死、炎症和纤维化评分均显著高于对照组（P < 0.01）。组织病理学参数与MR-HLP参数之间存在强相关性（r = 0.775，P < 0.01），其中脂肪变性和纤维化均与fmono呈正相关，与fdi呈负相关。然而，坏死和炎症与任何MR-HLP参数均无相关性。在非酒精性脂肪性肝病中，肝脏脂质组成似乎随着脂肪变性和纤维化的严重程度而改变，并且这些变化可以通过9.4 T场强下的MR-HLP方法在体内进行描绘。因此，虽然MR-HLP不太可能有助于区分早期脂肪性肝炎和单纯性脂肪变性，但这些发现总体上支持了高场强下体内MR-HLP在非酒精性脂肪性肝病中的潜在适用性。
