Food Biochemistry Laboratory, Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
Tsukuba Life Science Innovation Program (T-LSI), University of Tsukuba, Tsukuba, Ibaraki, Japan.
Biosci Biotechnol Biochem. 2021 Oct 21;85(11):2311-2321. doi: 10.1093/bbb/zbab151.
Maslinic acid, a naturally occurring pentacyclic triterpene in more than 30 plants (including olives), reportedly increases human muscle mass and muscle strength; however, the underlying molecular mechanism remains unknown. C57BL/6J mice were fed a standard diet or supplemented with 0.27% maslinic acid for 4 weeks, and their skeletal muscle mass was measured. Mice that consumed maslinic acid displayed significant increases in gastrocnemius and soleus muscle mass. Cultured mouse-C2C12 skeletal muscle cells were treated with mammalian target of rapamycin complex 1 (mTORC1) or protein kinase b (Akt) inhibitor, and protein synthesis was quantified. Maslinic acid accelerated protein synthesis via mTORC1 activation independent of Akt. Furthermore, maslinic acid activated human Takeda G protein-coupled receptor 5 (TGR5) more strongly than mouse TGR5, augmenting the expression of several genes related to muscular hypertrophy. Maslinic acid activated mTORC1 and human TGR5, implying its contribution to human muscular hypertrophy through these effects.
齐墩果酸是一种存在于 30 多种植物（包括橄榄）中的天然五环三萜，据报道可增加人体肌肉质量和肌肉力量；然而，其潜在的分子机制尚不清楚。C57BL/6J 小鼠喂食标准饮食或补充 0.27%齐墩果酸 4 周，测量其骨骼肌质量。摄入齐墩果酸的小鼠腓肠肌和比目鱼肌质量显著增加。用雷帕霉素靶蛋白复合物 1（mTORC1）或蛋白激酶 b（Akt）抑制剂处理培养的小鼠 C2C12 骨骼肌细胞，并定量蛋白质合成。齐墩果酸通过 mTORC1 激活加速蛋白质合成，而不依赖 Akt。此外，齐墩果酸激活人 Takeda G 蛋白偶联受体 5（TGR5）的作用强于鼠 TGR5，增强了与肌肉肥大相关的几个基因的表达。齐墩果酸激活 mTORC1 和人 TGR5，暗示其通过这些作用对人体肌肉肥大的贡献。
