Department of Radiology, the Third Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, 510150, China.
MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry, Sun Yat-sen University, Guangzhou, 510275, China.
Angew Chem Int Ed Engl. 2021 Oct 25;60(44):23608-23613. doi: 10.1002/anie.202110351. Epub 2021 Sep 30.
Herein, we report the first example of using mesoporous hydrogen-bonded organic frameworks (MHOFs) as the protecting scaffold to organize a biocatalytic cascade. The confined microenvironment of MHOFs has robust and large transport channels, allowing the efficient transport of a wide range of biocatalytic substrates. This new MHOF-confined cascade system shows superior activity, extended scope of catalytic substrates, and ultrahigh stability that enables the operation of complex chemical transformations in a porous carrier. In addition, the advantages of MHOF-confined cascades system for point-of-care biosensing are also demonstrated. This study highlights the advantages of HOFs as scaffold for multiple enzyme assemblies, which has huge potential for mimicking complex cellular transformation networks in a controllable manner.
在此，我们报告了首例使用介孔氢键有机骨架（MHOFs）作为保护支架来组织生物催化级联的案例。MHOFs 的受限微环境具有强大且大的传输通道，允许广泛的生物催化底物的有效传输。这种新的 MHOF 限域级联系统表现出优异的活性、扩展的催化底物范围和超高的稳定性，使复杂的化学转化能够在多孔载体中进行。此外，还展示了 MHOF 限域级联系统在即时生物传感方面的优势。本研究强调了 HOFs 作为多酶组装支架的优势，为以可控方式模拟复杂的细胞转化网络提供了巨大潜力。
