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酶与氢键有机框架生物复合材料的蒸汽辅助机械化学合成

Vapor-Assisted Mechanochemical Synthesis of Enzyme and Hydrogen-Bonded Organic Framework Biocomposites.

作者信息

Hafner Michael R, Pantalon Juraj Natalija, Flint Kate, Wiltsche Helmar, Wolinski Heimo, Amenitsch Heinz, Doonan Christian J, Užarević Krunoslav, Carraro Francesco

机构信息

Institute of Physical and Theoretical Chemistry, Graz University of Technology, Stremayrgasse 9, Graz, 8010, Austria.

Ruđer Bošković Institute, Bijenička c. 54, Zagreb, 10000, Croatia.

出版信息

Small. 2025 Aug;21(33):e2504744. doi: 10.1002/smll.202504744. Epub 2025 Jun 25.

DOI:10.1002/smll.202504744
PMID:40560335
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12372444/
Abstract

Hydrogen-bonded Organic Frameworks (HOFs) emerged as a matrix for preparing highly active and stable enzyme biocomposites. Conventional biocompatible synthetic procedures in solutions, however, suffer from issues related to competition with the solvent molecules and inhomogeneous loading of the enzyme. Here, it is demonstrated that a combination of mechanochemistry and accelerated aging can be used to synthesize Hydrogen-bonded Organic Framework (HOF) biocomposites with improved enzyme loading, activity, and protection. Advanced characterization techniques, including in situ Wide-Angle X-ray Scattering and Transmission Electron Microscopy, provide insights into these biocomposites' formation mechanisms and structural properties. A comparative analysis with biocomposites prepared via conventional solution synthesis reveals that vapor-induced growth enhances protein loading, ensures a more homogeneous enzyme distribution, and improves protective properties due to distinct growth mechanisms and kinetics. This simple and green synthetic approach offers a viable alternative to innovative HOF-based composite materials.

摘要

氢键有机框架（HOFs）作为一种用于制备高活性和稳定酶生物复合材料的基质而出现。然而，溶液中的传统生物相容性合成方法存在与溶剂分子竞争以及酶负载不均匀等问题。在此，证明了机械化学和加速老化相结合可用于合成具有改善的酶负载、活性和保护性能的氢键有机框架（HOF）生物复合材料。先进的表征技术，包括原位广角X射线散射和透射电子显微镜，为这些生物复合材料的形成机制和结构性质提供了深入了解。与通过传统溶液合成制备的生物复合材料的对比分析表明，气相诱导生长提高了蛋白质负载量，确保了更均匀的酶分布，并由于独特的生长机制和动力学而改善了保护性能。这种简单且绿色的合成方法为创新的基于HOF的复合材料提供了一种可行的替代方案。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a30/12372444/4f6fedf126c2/SMLL-21-2504744-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a30/12372444/9854f21fb237/SMLL-21-2504744-g008.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a30/12372444/d20d77bf5aee/SMLL-21-2504744-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a30/12372444/00ccf6e153f7/SMLL-21-2504744-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a30/12372444/1f35f7420ab7/SMLL-21-2504744-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a30/12372444/15cbb8de2e16/SMLL-21-2504744-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a30/12372444/4f6fedf126c2/SMLL-21-2504744-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a30/12372444/9854f21fb237/SMLL-21-2504744-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a30/12372444/9a12bc39baf4/SMLL-21-2504744-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a30/12372444/8da0f1f88a6f/SMLL-21-2504744-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a30/12372444/d20d77bf5aee/SMLL-21-2504744-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a30/12372444/00ccf6e153f7/SMLL-21-2504744-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a30/12372444/1f35f7420ab7/SMLL-21-2504744-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a30/12372444/15cbb8de2e16/SMLL-21-2504744-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a30/12372444/4f6fedf126c2/SMLL-21-2504744-g007.jpg

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