Department of Pharmacognosy, College of Pharmacy, Taif University, PO Box 11099, Taif 21944, Saudi Arabia.
Department of Pharmacognosy and Phytochemistry, School of Pharmaceutical Education & Research, Jamia Hamdard, New Delhi, India.
J AOAC Int. 2022 Feb 4;105(1):267-271. doi: 10.1093/jaoacint/qsab113.
Adhatoda vasica L. is a medicinal plant, known as Malabar nut in English, belonging to the family Acanthaceae. It has been used traditionally to treat respiratory disorders like severe coughs, colds, chronic bronchitis, asthma, tuberculosis, and other illnesses. The multifunctional range of bioactives found in it has piqued the interest of pharmaceutical companies, who are looking for more evidence-based ways to develop new formulations.
To analyse the A. vasica leaves by GC-MS technique and evaluation of its antioxidant activity.
Methanolic extract of A. vasica L. (MEAV) leaves was analyzed by GC-MS for identification and characterization of its bioactives and traditional therapeutic claims. A widely anticipated 2,2-diphenyl-1-picrylhydrazyl (DPPH) method was used to determine the antioxidant activity of MEAV.
The major compounds revealed in MEAV leaves are: 1,3,5-triazine-2,4,6-triamine (3.06%); 2,3-dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-one (5.35%); 5-hydroxymethylfurfural (16.82%); 2-butylphenol (6.85%); 3,4-dihydroxy-5-methyl-dihydro-furan-2-on (2.5%); 2(OR 3)-(1,1-dimethylethyl)-4-methoxyphenol (3.52%); megastigmatrienone 3 (1.02%); tetradecanoic acid (1.52%); vomifoliol (0.58%); oxalic acid, cyclobutylhexyl ester (6.03%); hexadecanoic acid (6.06%); 4-ethyl-2-oxo-2,5,6,7-tetrahydro-1H-cyclopenta[B]pyridine-3-carbonitrile (10.08%); phytol (2.01%); and vitamin E (3.18%). A significant reduction in free radicals against DPPH was observed, which revealed the antioxidant potential of MEAV.
MEAV consists of both polar and nonpolar components. GC-MS analysis was used to identify these compounds. The current work validates that the antioxidant activity of MEAV is attributed to the presence of compounds such as vitamin E and terpenes.
This work validates the antioxidant activity of methanolic extract of A. vasica attributed to the presence of compounds like vitamin E and terpenes.
印度大麻是一种药用植物，英文名为 Malabar nut，属于爵床科。它传统上被用于治疗呼吸系统疾病，如严重咳嗽、感冒、慢性支气管炎、哮喘、肺结核和其他疾病。其具有多种生物活性，引起了制药公司的兴趣，他们正在寻找更多基于证据的方法来开发新的配方。
通过 GC-MS 技术分析印度大麻叶，并评估其抗氧化活性。
用甲醇提取印度大麻叶（MEAV），通过 GC-MS 分析其生物活性成分和传统治疗功效。采用广泛预期的 2,2-二苯基-1-苦基肼（DPPH）法测定 MEAV 的抗氧化活性。
在 MEAV 叶片中发现的主要化合物有：1,3,5-三嗪-2,4,6-三胺（3.06%）；2,3-二氢-3,5-二羟基-6-甲基-4H-吡喃-4-酮（5.35%）；5-羟甲基糠醛（16.82%）；2-丁基苯酚（6.85%）；3,4-二羟基-5-甲基-二氢呋喃-2-酮（2.5%）；2（或 3）-（1,1-二甲基乙基）-4-甲氧基苯酚（3.52%）；大柱晶酮 3（1.02%）；十四烷酸（1.52%）；vomifoliol（0.58%）；草酸环己基丁基酯（6.03%）；十六烷酸（6.06%）；4-乙基-2-氧代-2,5,6,7-四氢-1H-环戊并[B]吡啶-3-甲腈（10.08%）；叶绿醇（2.01%）；和维生素 E（3.18%）。观察到 MEAV 对自由基有显著的清除作用，表明其具有抗氧化潜力。
MEAV 既含有极性成分，也含有非极性成分。采用 GC-MS 分析对这些化合物进行了鉴定。本研究证实，MEAV 的抗氧化活性归因于维生素 E 和萜烯等化合物的存在。
本研究证实，MEAV 的抗氧化活性归因于维生素 E 和萜烯等化合物的存在。
