Solís-Lemus José Alonso, Stramer Brian, Slabaugh Greg, Reyes-Aldasoro Constantino Carlos
School of Mathematics, Computer Science and Engineering, City, University of London, London EC1V 0HB, UK.
Randall Division of Cell & Molecular Biophysics, King's College London, London WC2R 2LS, UK.
J Imaging. 2019 Jan 14;5(1):17. doi: 10.3390/jimaging5010017.
This paper presents a novel software framework, called macrosight, which incorporates routines to detect, track, and analyze the shape and movement of objects, with special emphasis on macrophages. The key feature presented in macrosight consists of an algorithm to assess the changes of direction derived from cell-cell contact, where an interaction is assumed to occur. The main biological motivation is the determination of certain cell interactions influencing cell migration. Thus, the main objective of this work is to provide insights into the notion that interactions between cell structures cause a change in orientation. Macrosight analyzes the change of direction of cells before and after they come in contact with another cell. Interactions are determined when the cells overlap and form of two or more cells. The framework integrates a segmentation technique capable of detecting overlapping cells and a tracking framework into a tool for the analysis of the trajectories of cells before and after they overlap. Preliminary results show promise into the analysis and the hypothesis proposed, and lays the groundwork for further developments. The extensive experimentation and data analysis show, with statistical significance, that under certain conditions, the movement changes before and after an interaction are different from movement in controlled cases.
本文介绍了一种名为宏视（Macrosight）的新型软件框架，它包含用于检测、跟踪和分析物体形状及运动的程序，尤其侧重于巨噬细胞。宏视所呈现的关键特性包括一种算法，用于评估假定发生相互作用时源自细胞间接触的方向变化。主要的生物学动机是确定影响细胞迁移的某些细胞间相互作用。因此，这项工作的主要目标是深入了解细胞结构之间的相互作用会导致方向改变这一概念。宏视分析细胞在与另一个细胞接触前后的方向变化。当细胞重叠并形成两个或更多细胞的组合时，相互作用得以确定。该框架将能够检测重叠细胞的分割技术和一个跟踪框架集成到一个工具中，用于分析细胞重叠前后的轨迹。初步结果显示了对所提出的分析和假设的前景，并为进一步发展奠定了基础。广泛的实验和数据分析具有统计学意义地表明，在某些条件下，相互作用前后的运动变化与受控情况下的运动不同。
