Center for Public Health Genomics, School of Medicine, University of Virginia, Charlottesville, VA 22908 USA.
Department of Biochemistry and Molecular Genetics, School of Medicine, University of Virginia, Charlottesville, VA 22908 USA.
STAR Protoc. 2021 Aug 21;2(3):100768. doi: 10.1016/j.xpro.2021.100768. eCollection 2021 Sep 17.
This protocol describes the application of the "omnigenic" model of the genetic architecture of complex traits to identify novel "core" genes influencing a disease-associated phenotype. Core genes are hypothesized to directly regulate disease and may serve as therapeutic targets. This protocol leverages GWAS data, a co-expression network, and publicly available data, including the GTEx database and the International Mouse Phenotyping Consortium Database, to identify modules enriched for genes with "core-like" characteristics. For complete details on the use and execution of this protocol, please refer to Sabik et al. (2020).
本方案描述了将复杂特征遗传结构的“全能性”模型应用于鉴定影响疾病相关表型的新“核心”基因。核心基因被假设为直接调控疾病，可能作为治疗靶点。本方案利用 GWAS 数据、共表达网络以及包括 GTEx 数据库和国际小鼠表型联盟数据库在内的公开数据，鉴定富含具有“核心样”特征的基因的模块。如需详细了解本方案的使用和执行，请参考 Sabik 等人（2020 年）。
