CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, China.
College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China.
Phytochem Anal. 2022 Mar;33(2):272-285. doi: 10.1002/pca.3086. Epub 2021 Sep 1.
Portulaca oleracea is a commonly used nutritional vegetable and traditional herbal medicine with plenty of nutrients and manifold pharmacological activities. However, the potential active ingredients for its remarkable antioxidant, hypoglycemic and hypolipidemic activities remain unexplored.
The present study aims to systematically evaluate the antioxidant activities of different extracts of P. oleracea and screen bioactive ligands that can interact with α-glucosidase, pancreatic lipase, and superoxide dismutase (SOD).
In this research, the antioxidant activities of different parts of P. oleracea and their corresponding total phenolic content (TPC) and total flavonoid content (TFC) were systematically determined. Subsequently, a multi-target affinity ultrafiltration method was developed using affinity ultrafiltration with SOD, α-glucosidase, and pancreatic lipase coupled to liquid chromatography-mass spectrometry (UF-LC-MS). Later, molecular docking was used to further investigate the possible interaction mechanism between these ligands and target enzymes.
Among them, the ethyl acetate (EA) fraction showed the highest antioxidant activity along with the highest TPC and TFC, and four compounds in the EA fraction were quickly retrieved as potential SOD, α-glucosidase, and pancreatic lipase ligands, respectively. Molecular docking revealed that these potential ligands exhibited strong binding ability and inhibitory activities on SOD, α-glucosidase, and pancreatic lipase.
The present study revealed that P. oleracea can be used as a functional food with excellent antioxidant, hypoglycemic and hypolipidemic effects. Meanwhile, the integrated strategy based on multi-target UF-LC-MS and molecular docking also provided a powerful tool and a multidimensional perspective for further exploration of active ingredients in P. oleracea responsible for the antioxidant, hypoglycemic and hypolipidemic activities.
马齿苋是一种常见的营养蔬菜和传统草药，含有丰富的营养成分和多种药理活性。然而，其具有显著抗氧化、降血糖和降血脂活性的潜在活性成分仍未被探索。
本研究旨在系统评价马齿苋不同提取物的抗氧化活性，并筛选能与α-葡萄糖苷酶、胰脂肪酶和超氧化物歧化酶（SOD）相互作用的生物活性配体。
在这项研究中，系统地测定了马齿苋不同部位及其相应总酚含量（TPC）和总黄酮含量（TFC）的抗氧化活性。随后，采用 SOD、α-葡萄糖苷酶和胰脂肪酶与液相色谱-质谱联用的亲和超滤法（UF-LC-MS）建立了一种多靶点亲和超滤法。之后，利用分子对接进一步研究了这些配体与靶酶之间可能的相互作用机制。
其中，乙酸乙酯（EA）部分表现出最高的抗氧化活性，同时具有最高的 TPC 和 TFC，并且从 EA 部分中快速检索到四种化合物，分别为潜在的 SOD、α-葡萄糖苷酶和胰脂肪酶配体。分子对接表明，这些潜在的配体对 SOD、α-葡萄糖苷酶和胰脂肪酶表现出强烈的结合能力和抑制活性。
本研究表明马齿苋可用作具有优异抗氧化、降血糖和降血脂作用的功能性食品。同时，基于多靶点 UF-LC-MS 和分子对接的综合策略也为进一步探索马齿苋中负责抗氧化、降血糖和降血脂活性的活性成分提供了有力的工具和多维视角。
