Key Laboratory of Delivery Systems of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, Jiangsu, China; College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China.
Key Laboratory of Delivery Systems of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, Jiangsu, China; College of Pharmacy, Jiangsu University, Zhenjiang 212013, Jiangsu, China.
J Ethnopharmacol. 2015 Feb 23;161:214-23. doi: 10.1016/j.jep.2014.12.002. Epub 2014 Dec 16.
Fresh Portulaca oleracea L. (family: Portulacaceae; POL) has been used as a folk medicine for the treatment of diabetes mellitus for a long time. More bioactive components with higher activity could be retained in fresh medicinal herbs compared to the dried ones. The present study was conducted to compare different antidiabetic activity between fresh and dried POL, including hypoglycemic and antioxidant activities both in vivo and in vitro. Furthermore, in order to explore which components were responsible for the antidiabetic activity, the difference on chemical components between fresh and dried POL was analyzed and compared.
Insulin-resistant HepG2 cells induced by insulin were used to evaluate the promoting effect of the fresh and dried POL on glucose utilization in vitro. Streptozotocin (STZ)-induced C57BL/6J diabetic mice were used to compare the differences on hypoglycemic and antioxidant activities of fresh and dried POL, including the fasting blood glucose, glucose tolerance, serum insulin level, malondialdehyde (MDA) level and superoxide dismutase (SOD) activity in vivo. UPLC/Q-TOF-MS method was performed to analyze the difference of antidiabetic components between fresh and dried POL.
Compared with the dried POL extract, the fresh POL extract significantly increased the consumption of extracellular glucose in insulin-resistant HepG2 cells (P<0.05). In STZ-induced C57BL/6J diabetic mice, both fresh and dried extracts decreased markedly the fasting blood glucose (FBG) levels, and improved significantly oral glucose tolerance test (OGTT), as well as enhanced significantly insulin secretion and antioxidative activities (P<0.05; P<0.01). Furthermore, the fresh extract showed stronger antidiabetic activity (P<0.05). The UPLC/Q-TOF-MS analysis results also revealed that the relative contents of polyphenols and alkaloids in the fresh herbs were more abundant than those in the dried POL.
Our results indicated that both fresh and dried POL possessed antidiabetic activities, besides stronger activity was observed in the fresh herb. These findings provided evidence for the application and development of fresh POL in the treatment of diabetes mellitus.
马齿苋（马齿苋科）作为一种民间药物，长期以来一直被用于治疗糖尿病。与干燥的草药相比，新鲜草药中可能保留了更多具有更高活性的生物活性成分。本研究旨在比较新鲜和干燥马齿苋之间不同的降血糖和抗氧化活性，包括体内和体外的降糖和抗氧化活性。此外，为了探讨哪些成分具有降血糖活性，分析和比较了新鲜和干燥马齿苋之间的化学成分差异。
使用胰岛素诱导的胰岛素抵抗 HepG2 细胞评估新鲜和干燥 POL 对体外葡萄糖利用的促进作用。使用链脲佐菌素（STZ）诱导的 C57BL/6J 糖尿病小鼠比较新鲜和干燥 POL 在降血糖和抗氧化活性方面的差异，包括体内空腹血糖、葡萄糖耐量、血清胰岛素水平、丙二醛（MDA）水平和超氧化物歧化酶（SOD）活性。采用 UPLC/Q-TOF-MS 法分析新鲜和干燥 POL 中降血糖成分的差异。
与干燥 POL 提取物相比，新鲜 POL 提取物显著增加了胰岛素抵抗 HepG2 细胞对细胞外葡萄糖的消耗（P<0.05）。在 STZ 诱导的 C57BL/6J 糖尿病小鼠中，新鲜和干燥提取物均显著降低了空腹血糖（FBG）水平，显著改善了口服葡萄糖耐量试验（OGTT），并显著增强了胰岛素分泌和抗氧化活性（P<0.05；P<0.01）。此外，新鲜提取物表现出更强的降血糖活性（P<0.05）。UPLC/Q-TOF-MS 分析结果还表明，新鲜草药中多酚和生物碱的相对含量比干燥 POL 更丰富。
我们的结果表明，新鲜和干燥的马齿苋均具有降血糖活性，且新鲜草药的活性更强。这些发现为新鲜马齿苋在治疗糖尿病中的应用和开发提供了依据。
