Department of General Surgery, Heping Hospital, Changzhi Medical College, Changzhi City, Shanxi Province, China.
Changzhi Medical College, Changzhi City, Shanxi Province, China.
Cancer Gene Ther. 2023 Apr;30(4):559-566. doi: 10.1038/s41417-021-00382-w. Epub 2021 Sep 1.
Accumulating research implicated that circular RNAs exhibited significant roles in cancer development. Nonetheless, the role regarding circPTPN22 in pancreatic cancer remains unclear. Expression of circPTPN22 in pancreatic cancer cell lines and normal cells was determined with quantitative real-time PCR (qRT-PCR). Cell counting kit-8 assay and colony formation assay were used to measure the proliferation of pancreatic cancer cells. RNA immunoprecipitation and Western blot were employed for investigation the binding between circPTPN22 and STAT3. circPTPN22 expression was highly upregulated in pancreatic cancer tissues and cell lines. Knockdown of circPTPN22 inhibited cell proliferation and attenuates pancreatic cancer immune microenvironment. Furthermore, STAT3 acetylation was involved in these effects. circPTPN22 promoted STAT3 acetylation via inhibiting STAT3/SIRT1 interaction. circPTPN22 attenuates pancreatic cancer immune microenvironment by promoting STAT3 acetylation via inhibiting STAT3/SIRT1 interaction.
越来越多的研究表明，环状 RNA 在癌症发展中具有重要作用。然而，circPTPN22 在胰腺癌中的作用尚不清楚。采用实时定量 PCR（qRT-PCR）检测胰腺癌细胞系和正常细胞中 circPTPN22 的表达。细胞计数试剂盒-8 检测和集落形成实验用于检测胰腺癌细胞的增殖。RNA 免疫沉淀和 Western blot 用于研究 circPTPN22 和 STAT3 之间的结合。circPTPN22 在胰腺癌组织和细胞系中高表达。circPTPN22 敲低抑制细胞增殖并减弱胰腺癌免疫微环境。此外，STAT3 乙酰化参与了这些效应。circPTPN22 通过抑制 STAT3/SIRT1 相互作用促进 STAT3 乙酰化。circPTPN22 通过抑制 STAT3/SIRT1 相互作用促进 STAT3 乙酰化，从而减弱胰腺癌免疫微环境。
