F-FDG PET/CT代谢参数与结直肠癌中EIF2S2表达状态相关。
F-FDG PET/CT metabolic parameters correlate with EIF2S2 expression status in colorectal cancer.
作者信息
Yang Jian-Wei, Yuan Ling-Ling, Gao Yan, Liu Xu-Sheng, Wang Yu-Jiao, Zhou Lu-Meng, Kui Xue-Yan, Li Xiao-Hui, Ke Chang-Bin, Pei Zhi-Jun
机构信息
Postgraduate Training Basement of Jinzhou Medical University, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, China.
Department of Nuclear Medicine and Institute of Anesthesiology and Pain, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, China.
出版信息
J Cancer. 2021 Aug 3;12(19):5838-5847. doi: 10.7150/jca.57926. eCollection 2021.
We sought to investigate whether the expression of the gene EIF2S2 is related to F-FDG PET/CT metabolic parameters in patients with colorectal cancer (CRC). The expression of EIF2S2 in CRC and its relationship with clinicopathological features were obtained through the ONCOMINE, UALCAN and GEPIA databases. EIF2S2 and GLUT1 expression were examined by immunohistochemistry in 42 CRC patients undergoing preoperative PET-CT examination. Spearman correlation analysis was used to assess the relationship between EIF2S2 and GLUT1 levels and clinical parameters. Correlation analysis between EIF2S2 and Reactome-Glycolysis signatures was performed using GEPIA2. We describe the effect of EIF2S2 knockdown on lactate production and the mRNA levels of glycolysis-related genes in human colon cancer SW480 cells. Immunohistochemistry revealed an upregulation of EIF2S2 protein expression in tumor tissues of colorectal cancer patients, which is consistent with the significant upregulation of EIF2S2 transcript levels in the database. These colorectal cancer patients included 24 cases of colon cancer and 18 cases of rectal cancer, ranging in age from 31 to 78 years. The transcription was significantly related to histological subtypes and TP53 mutations (P <0.05). The value of SUVmax in CRC significantly correlated with the expression of EIF2S2 (rho = 0.462, P <0.01). Although SUVmax and SUVmean was not correlate with the expression of GLUT1 (P <0.05), a significant correlation was observed between the expression of GLUT1 and the volumetric PET parameters, such as MTV and TLG ( < 0.01). GLUT1 expression in CRC was positively correlated with EIF2S2 status (rho = 0.470, P <0.01). In SW480 cells, RNAi-mediated depletion of EIF2S2 inhibited lactic acid production (P <0.05) and SLC2A1, SLC2A3, SLC2A10, HK2, PKM2, LDHA mRNA level (P <0.01). Primary CRC FDG uptake is strongly associated with the overexpression of EIF2S2, and EIF2S2 may promote glycolysis in CRC by mediating GLUT1.
我们试图研究基因EIF2S2的表达是否与结直肠癌（CRC）患者的F-FDG PET/CT代谢参数相关。通过ONCOMINE、UALCAN和GEPIA数据库获取EIF2S2在结直肠癌中的表达及其与临床病理特征的关系。对42例接受术前PET-CT检查的结直肠癌患者进行免疫组织化学检测EIF2S2和GLUT1的表达。采用Spearman相关性分析评估EIF2S2和GLUT1水平与临床参数之间的关系。使用GEPIA2进行EIF2S2与Reactome-糖酵解特征之间的相关性分析。我们描述了EIF2S2敲低对人结肠癌SW480细胞乳酸产生和糖酵解相关基因mRNA水平的影响。免疫组织化学显示结直肠癌患者肿瘤组织中EIF2S2蛋白表达上调，这与数据库中EIF2S2转录水平的显著上调一致。这些结直肠癌患者包括24例结肠癌和18例直肠癌，年龄在31至78岁之间。转录与组织学亚型和TP53突变显著相关（P<0.05）。结直肠癌中SUVmax值与EIF2S2的表达显著相关（rho = 0.462，P<0.01）。虽然SUVmax和SUVmean与GLUT1的表达无相关性（P<0.05），但观察到GLUT1的表达与体积PET参数如MTV和TLG之间存在显著相关性（<0.01）。结直肠癌中GLUT1的表达与EIF2S2状态呈正相关（rho = 0.470，P<0.01）。在SW480细胞中，RNAi介导的EIF2S2缺失抑制了乳酸产生（P<0.05）以及SLC2A1、SLC2A3、SLC2A10、HK2、PKM2、LDHA的mRNA水平（P<0.01）。原发性结直肠癌FDG摄取与EIF2S2的过表达密切相关，并且EIF2S2可能通过介导GLUT1促进结直肠癌中的糖酵解。
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