Xiao Shu, Yamada Ryo, Zhou Carol
Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, Virginia, USA.
Department of Electrical and Computer Engineering, Old Dominion University, Norfolk, Virginia, USA.
Bioelectricity. 2020 Dec 1;2(4):382-390. doi: 10.1089/bioe.2020.0024. Epub 2020 Dec 16.
A method that utilizes nanosecond bipolar cancellation (BPC) near a quadrupole electrodes to suppress a biological response but cancels the distal BPC at the quadrupole center, i.e., cancellation of cancellation (CANCAN), may allow for a remote focused stimulation at the quadrupole center. The primary object of this study was to outline the requirement of the CANCAN implementation and select an effective quadrupole configuration. We have studied three quadrupole electrode configurations, a rod quadrupole, a plate quadrupole (Plate-Q), and a resistor quadrupole. The pulse shapes of electric fields include monophasic pulses, cancellation pulses, and additive pulses. The Plate-Q appears the best for CANCAN as it shows the highest percentage of cancellation pulses among all pulse shapes, allowing for the best spatial focus. For the region of interest characterized in the Plate-Q configuration, the maximum magnitude of bipolar field is twice as that of the unipolar field, which allows for the CANCAN demonstration that involves membrane electropermeabilization.
一种利用四极电极附近的纳秒双极抵消（BPC）来抑制生物反应，但在四极中心消除远端BPC，即抵消抵消（CANCAN）的方法，可能允许在四极中心进行远程聚焦刺激。本研究的主要目的是概述CANCAN实施的要求并选择有效的四极配置。我们研究了三种四极电极配置，棒状四极、平板四极（Plate-Q）和电阻四极。电场的脉冲形状包括单相脉冲、抵消脉冲和叠加脉冲。Plate-Q似乎最适合CANCAN，因为在所有脉冲形状中它显示出最高比例的抵消脉冲，从而实现最佳的空间聚焦。对于以Plate-Q配置为特征的感兴趣区域，双极场的最大幅度是单极场的两倍，这使得涉及膜电通透化的CANCAN演示成为可能。
