Colditz I G
C.S.I.R.O. Division of Animal Health, Armidale, N.S.W., Australia.
Immunol Cell Biol. 1987 Dec;65 ( Pt 6):437-41. doi: 10.1038/icb.1987.52.
In non-lactating ewes, intramammary infusion of endotoxin provokes an intense but transient inflammatory response as assessed by mammary lavage. The variability of the method for sampling the leucocytes in mammary secretions was examined. Initial lavage with 20 ml pyrogen-free saline recovered 83.0 +/- 2.8 and 84.4 +/- 3.0% of the total leucocyte numbers harvested by three serial washes of quiescent and inflamed glands, respectively. Lavage of glands prior to infusion of endotoxin did not affect the magnitude of the subsequent inflammatory response. Three treatments at 3.5 day intervals with the glucocorticoid, dexamethasone, which inhibits the inflammatory response to intramammary infusion of antigen in systemically immunized ewes, did not affect the inflammatory response to endotoxin. However, the variability of the inflammatory response to activated complement was greatly increased in dexamethasone-treated ewes; this effect was attributed to the induction of lactation which results in loss of sensitivity of the gland to the inflammatory activity of activated complement. The results indicated that the macrophages and lymphocytes which are present in mammary secretions in large numbers, and dexamethasone-sensitive lymphocytes in the gland, played a trivial role in the induction of inflammatory responses to endotoxin.
在非泌乳母羊中，通过乳腺灌洗评估发现，乳腺内注入内毒素会引发强烈但短暂的炎症反应。研究了乳腺分泌物中白细胞采样方法的变异性。用20毫升无热原生理盐水进行初次灌洗，分别回收了通过对静止和发炎腺体进行三次连续冲洗所收获的白细胞总数的83.0±2.8%和84.4±3.0%。在内毒素注入前对腺体进行灌洗并不影响随后炎症反应的程度。以3.5天的间隔用糖皮质激素地塞米松进行三次处理，地塞米松可抑制全身免疫母羊对乳腺内注入抗原的炎症反应，但并不影响对内毒素的炎症反应。然而，在地塞米松处理的母羊中，对活化补体的炎症反应变异性大大增加；这种效应归因于泌乳的诱导，这导致腺体对活化补体的炎症活性敏感性丧失。结果表明，大量存在于乳腺分泌物中的巨噬细胞和淋巴细胞，以及腺体中对地塞米松敏感的淋巴细胞，在内毒素炎症反应的诱导中起的作用微不足道。
