Institute of Nanoscience and Nanotechnology, University of Kashan, Kashan, Islamic Republic of Iran.
Department of Analytical Chemistry, Faculty of Chemistry, University of Kashan, Kashan, Islamic Republic of Iran.
Int J Biol Macromol. 2015;78:130-6. doi: 10.1016/j.ijbiomac.2015.02.042. Epub 2015 Mar 4.
Magnetite nanoparticles were synthesized by coprecipitation under ultrasonication followed by coating with chitosan. Polyvinyl alcohol (PVA) is then combined with the chitosan that coated the magnetite nanoparticles. The combination occurs by hydrogen binding and ionic cross-linking of the amino and hydroxyl groups of chitosan and PVA respectively. The magnetite nanoparticles have an average size of 10.62 nm that was confirmed by TEM. The VSM measurements showed that nanoparticles were superparamagnetic. The coatings on the core nanoparticles were estimated by AAS and the attachments of coating to the nanoparticles were confirmed by FT-IR analysis. Physicochemical properties of nanoparticles were measured by DLS and zeta potential. Naked magnetite, chitosan and PVA coating have zeta potential of +36.4, +48.1 and -12.5 mV respectively. The unspecific adsorption and interaction between nanoparticles and bovine serum albumin (BSA) were investigated systematically by UV-vis spectroscopy method. The nanoparticles that were modified by PVA present low protein adsorption, which makes them a practical choice for preventing opsonization in clinical application and drug delivery.
通过超声辅助共沉淀法合成了磁铁矿纳米粒子，然后用壳聚糖进行包覆。然后将聚乙烯醇（PVA）与包覆在磁铁矿纳米粒子上的壳聚糖结合。这种结合是通过壳聚糖和 PVA 的氨基和羟基的氢键和离子交联来实现的。TEM 证实纳米粒子的平均尺寸为 10.62nm。VSM 测量表明纳米粒子具有超顺磁性。通过 AAS 估计了核纳米粒子上的涂层，并通过 FT-IR 分析证实了涂层对纳米粒子的附着。通过 DLS 和 ζ 电位测量了纳米粒子的物理化学性质。裸露的磁铁矿、壳聚糖和 PVA 涂层的 ζ 电位分别为+36.4、+48.1 和-12.5mV。通过紫外可见光谱法系统地研究了纳米粒子与牛血清白蛋白（BSA）之间的非特异性吸附和相互作用。通过 PVA 修饰的纳米粒子表现出低蛋白吸附，这使得它们成为临床应用和药物输送中防止调理作用的实用选择。
