Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA 94550, USA.
Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA 94550, USA.
Curr Opin Pharmacol. 2021 Oct;60:255-260. doi: 10.1016/j.coph.2021.08.003. Epub 2021 Sep 1.
Recent advances in microphysiological systems have made significant strides to include design features that reconstruct key elements found in the brain, and in parallel advance technologies to detect the activity of electrogenic cells that form neural networks. In particular, three-dimensional multielectrode arrays (3D MEAs) are being developed with increasing levels of spatial and temporal precision, difficult to achieve with current 2D MEAs, insertable MEA probes, and/or optical imaging of calcium dynamics. Thus, providing a means to monitor the flow of neural network activity within all three dimensions (X, Y, and Z) of the engineered tissue. In the last 6 years, 3D MEAs, using either bottom-up or top-down designs, have been developed to overcome the current technical challenges in monitoring the functionality of the in vitro systems. Herein, we will report on the design and application of novel 3D MEA prototypes for probing neural activity throughout the 3D neural tissue.
近年来，微生理系统的发展取得了重大进展，包括设计出能够再现大脑关键元素的功能，同时也在推进能够检测形成神经网络的发电细胞活动的技术。特别是，正在开发具有越来越高的空间和时间精度的三维多电极阵列 (3D MEA)，这很难通过当前的 2D MEA、可插入 MEA 探头和/或钙动态的光学成像来实现。因此，提供了一种监测工程组织中所有三个维度（X、Y 和 Z）内神经网络活动的方法。在过去的 6 年中，已经开发出了使用自下而上或自上而下设计的 3D MEA，以克服当前监测体外系统功能的技术挑战。在此，我们将报告用于探测整个 3D 神经组织中的神经活动的新型 3D MEA 原型的设计和应用。
