Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, GA.
Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, GA.
Am J Infect Control. 2022 Jan;50(1):54-60. doi: 10.1016/j.ajic.2021.08.027. Epub 2021 Sep 2.
Healthcare-associated infections are a significant economic burden and cause of avoidable morbidity and mortality within healthcare systems. The contribution of environmental contamination to healthcare-associated infection transmission has been recognized, but the mechanisms by which transmission occurs are still being investigated. The objective of this study was to characterize the microbial communities of disinfected, non-critical healthcare surfaces using next generation sequencing technology.
Composite environmental surface samples were from high-touch surfaces in rooms of patients isolated for infections with multidrug-resistant organisms during their hospitalization. Information on the disinfectant product used and cleaning type (routine or terminal) was collected. 16S rRNA gene amplicon sequencing and analysis were performed. Community analysis was conducted to determine the bacterial composition and compare the detection of target pathogens by culture from 94 Contact Precaution rooms.
Overall percent agreement between culture and sequence methods ranged from 52%-88%. A significant difference was observed in bacterial composition between rooms cleaned with bleach and those cleaned with a quaternary ammonium compound for composite 2 (overbed table, intravenous pole, and inner room door handle) (ANOSIM R = 0.66, P = .005) but not composite 1 (bed rails, television remote control unit, call buttons, and telephone).
Surfaces in bleach-cleaned rooms contained a higher proportion of gram-positive microbiota, whereas rooms cleaned with quaternary ammonium compound contained a higher proportion of gram-negative microbiota, suggesting disinfectant products may impact the healthcare environment microbiome.
医疗保健相关感染是医疗系统中重大的经济负担和可避免发病率和死亡率的原因。环境污染物对医疗保健相关感染传播的贡献已得到承认，但传播发生的机制仍在研究中。本研究的目的是使用下一代测序技术对消毒的非关键医疗保健表面的微生物群落进行特征描述。
复合环境表面样本来自感染多药耐药菌的患者隔离病房中高接触表面的房间。收集了有关使用的消毒剂产品和清洁类型（常规或终末）的信息。进行了 16S rRNA 基因扩增子测序和分析。进行群落分析以确定细菌组成，并比较 94 间接触预防房间中培养物检测到的目标病原体。
培养与测序方法之间的总体百分比一致性在 52%-88%之间。用含氯漂白剂清洁的房间和用季铵化合物清洁的房间之间的复合 2（床上桌、静脉杆和内室门把手）的细菌组成存在显著差异（非参数相似性分析 R=0.66，P=0.005），但复合 1（床栏、电视遥控器单元、呼叫按钮和电话）则没有。
用含氯漂白剂清洁的房间中含有更高比例的革兰氏阳性微生物群落，而用季铵化合物清洁的房间中含有更高比例的革兰氏阴性微生物群落，这表明消毒剂产品可能会影响医疗保健环境微生物组。
