Du Chunyang, Ren Yunzhuo, Li Guixin, Yang Yan, Yan Zhe, Yao Fang
Key Laboratory of Kidney Diseases of Hebei Province, Department of Pathology, Hebei Medical University, Shijiazhuang, China.
Department of Burn, The Second Hospital of Hebei Medical University, Shijiazhuang, China.
Front Med (Lausanne). 2021 Aug 17;8:657614. doi: 10.3389/fmed.2021.657614. eCollection 2021.
Years of research revealed that crosstalk extensively existed among kidney cells, cell factors and metabolites and played an important role in the development of diabetic kidney disease (DKD). In the last few years, single-cell RNA sequencing (scRNA-seq) technology provided new insight into cellular heterogeneity and genetic susceptibility regarding DKD at cell-specific level. The studies based on scRNA-seq enable a much deeper understanding of cell-specific processes such as interaction between cells. In this paper, we aim to review recent progress in single cell transcriptomic analyses of DKD, particularly highlighting on intra- or extra-glomerular cell crosstalk, cellular targets and potential therapeutic strategies for DKD.
多年的研究表明，肾脏细胞、细胞因子和代谢物之间广泛存在相互作用，并在糖尿病肾病（DKD）的发展中发挥重要作用。在过去几年中，单细胞RNA测序（scRNA-seq）技术为在细胞特异性水平上了解DKD的细胞异质性和遗传易感性提供了新的视角。基于scRNA-seq的研究能够更深入地了解细胞特异性过程，如细胞间的相互作用。在本文中，我们旨在综述DKD单细胞转录组分析的最新进展，特别强调肾小球内或肾小球外细胞间的相互作用、细胞靶点以及DKD的潜在治疗策略。
