Wu Haigang, Wu Xian, Huang Li, Ruan Chongmei, Liu Jinni, Chen Xiaoqing, Liu Jicheng, Luo Houqing
College of Animal Science and Veterinary Medicine, Xinyang Agriculture and Forestry University, Xinyang, China.
College of Animal Science, Wenzhou Vocational College of Science and Technology, Wenzhou, China.
Front Vet Sci. 2021 Aug 18;8:702885. doi: 10.3389/fvets.2021.702885. eCollection 2021.
The intestinal flora is a micro-ecosystem that is closely linked to the overall health of the host. We examined the diversity and abundance of intestinal microorganisms in mice following the administration of andrographolide, a component of the Chinese medical herb . Administration of andrographolide produces multiple beneficial effects including anti-inflammatory, antiviral and antibacterial effects but whether it directly influences the gut microbiota is not known. This study investigated whether the oral administration of andrographolide influences the intestinal microbiota and was compared with amoxicillin treatment as a positive control and water only as a negative control. We examined 21 cecal samples and conducted a high-throughput sequencing analysis based on V3-V4 variable region of the 16S rDNA genes. We found that the diversity and abundance of mouse gut microbiota decreased in direct proportion with the amoxicillin dose whereas andrographolide administration did not affect intestinal microbial community structure. The composition of intestinal microbes following andrographolide treatment was dominated by the while dominated the amoxicillin treatment group compared with the negative controls. Specifically, the and were enriched with andrographolide administration while and significantly increased in the amoxicillin test groups. Amoxicillin administration altered the microbial community composition and structure by increasing the proportion of pathogenic to beneficial bacteria whereas andrographolide administration led to increases in the proportions and abundance of beneficial bacteria. This study provides a theoretical basis for finding alternatives to antibiotics to decrease bacterial resistance and restore intestinal floral imbalances.
肠道菌群是一个与宿主整体健康密切相关的微生态系统。我们研究了给予穿心莲内酯（一种中草药成分）后小鼠肠道微生物的多样性和丰度。穿心莲内酯具有多种有益作用，包括抗炎、抗病毒和抗菌作用，但它是否直接影响肠道微生物群尚不清楚。本研究调查了口服穿心莲内酯是否会影响肠道微生物群，并与作为阳性对照的阿莫西林治疗以及仅作为阴性对照的水进行了比较。我们检查了21个盲肠样本，并基于16S rDNA基因的V3 - V4可变区进行了高通量测序分析。我们发现，小鼠肠道微生物群的多样性和丰度与阿莫西林剂量成反比，而给予穿心莲内酯并不影响肠道微生物群落结构。与阴性对照相比，穿心莲内酯治疗后肠道微生物的组成以[具体菌群名称1]为主，而阿莫西林治疗组以[具体菌群名称2]为主。具体而言，给予穿心莲内酯后[具体菌群名称3]和[具体菌群名称4]富集，而在阿莫西林试验组中[具体菌群名称5]和[具体菌群名称6]显著增加。阿莫西林给药通过增加致病菌与有益菌的比例改变了微生物群落组成和结构，而穿心莲内酯给药导致有益菌的比例和丰度增加。本研究为寻找抗生素替代品以降低细菌耐药性和恢复肠道菌群失衡提供了理论依据。
