Taqvi Shagufta, Ahmed Bhat Eijaz, Sajjad Nasreena, Sabir Jamal S M, Qureshi Aleem, Rather Irfan A, Rehman Suriya
Department of Biochemistry and Biotechnology, Annamalai University, Chidambaram, Tamil Nadu, India.
Life Science Institute, Zhejiang University, Hangzhou, Zhejiang 310058, PR China.
Saudi J Biol Sci. 2021 Mar;28(3):1795-1800. doi: 10.1016/j.sjbs.2020.12.023. Epub 2020 Dec 19.
The overproduction of reactive oxygen species (ROS) causes oxidative stress, such as Hydrogen peroxide (HO). Acute oxidative stress is one of the main reasons for cell death. In this study, the antioxidant properties of vanillic acid- a polyphenolic compound was evaluated. Therefore, this study aims to check the effectiveness of vanillic acid in HO-induced oxidative stress in D. Mel-2 cell line. The efficacy was determined by biochemical tests to check the ROS production. The cytotoxicity of HO and vanillic acid was checked by MTT assay. The DNA fragmentation was visualized by gel electrophoresis. Protein biomarkers of oxidative stress were analyzed by western blotting. The results depict a promising antioxidant effect of vanillic acid. The IC value of vanillic acid and HO was found 250 μg/ml and 125 μg/ml, respectively. The catalase activity, SOF, GPx, and PC was seen less in HO treated group compared with the control and vanillic acid treated group. However, the TBRAS activity was hight in HO treated group. The effect of HO on DNA fragmentation was high as compared with vanillic acid-treated cells. The protein expression of Hsp70, IL-6 and iNOS was seen significant in a vanillic acid-treated group as compared with HO treated group. These results reinforce that at low concentration, vanillic acid could be used as an antioxidant agent in the food and pharmaceutical industries.
活性氧（ROS）的过量产生会导致氧化应激，如过氧化氢（HO）。急性氧化应激是细胞死亡的主要原因之一。在本研究中，对一种多酚化合物香草酸的抗氧化特性进行了评估。因此，本研究旨在检验香草酸在HO诱导的D. Mel - 2细胞系氧化应激中的有效性。通过生化试验检测ROS产生来确定其有效性。通过MTT法检测HO和香草酸的细胞毒性。通过凝胶电泳观察DNA片段化情况。通过蛋白质印迹法分析氧化应激的蛋白质生物标志物。结果显示香草酸具有良好的抗氧化作用。发现香草酸和HO的IC值分别为250μg/ml和125μg/ml。与对照组和香草酸处理组相比，HO处理组的过氧化氢酶活性、SOF、谷胱甘肽过氧化物酶（GPx）和PC较低。然而，HO处理组的TBRAS活性较高。与香草酸处理的细胞相比，HO对DNA片段化的影响更大。与HO处理组相比，香草酸处理组中热休克蛋白70（Hsp70）、白细胞介素 - 6（IL - 6）和诱导型一氧化氮合酶（iNOS）的蛋白质表达显著。这些结果表明，在低浓度下，香草酸可作为食品和制药行业的抗氧化剂。
