Tianjin Key Laboratory of Retinal Functions and Diseases, Tianjin Branch of National Clinical Research Center for Ocular Disease, Eye Institute and School of Optometry, Tianjin Medical University Eye Hospital, No. 251, FuKang Road, Nankai District, Tianjin 30384, China.
University of California Santa Cruz, 1156 High Street, Santa Cruz, CA 95064, USA.
J Immunol Res. 2021 Aug 25;2021:7377685. doi: 10.1155/2021/7377685. eCollection 2021.
The aim of this study was to explore the correlation between intraoperative hyperalgesia of the second eye and the dynamic changes of tumor necrosis factor (TNF)- and interleukin (IL)-1 levels in aqueous humor (AH) of the second eye and whole blood after the first eye cataract surgery. A rabbit model of monocular phacoemulsification was established by administration of 0.3% levofloxacin. Whole blood and AH samples from non-surgical eyes in the experimental group (n =25) and second eye in the blank control group (n =15) were obtained and corneal sensitivity was examined after surgery (1, 3, 7, 14, and 21 days postoperatively). TNF- and IL-1 levels in AH and TNF- mRNA and IL-1 mRNA levels in whole blood were measured. In a clinical study, 30 patients who underwent bilateral phacoemulsification within 1 month were divided into six groups in accordance with the operation intervals (1, 3, 7, 10, 14, and 21days). TNF- and IL-1 levels in AH were measured at the beginning of surgery and intraoperative pain was assessed immediately after surgery. Corneal sensitivity (F =244.910, P <0.05), TNF- and IL-1 levels in AH (F =184.200, 82.900, P <0.05) of non-surgical eyes and in whole blood (F =272.800, 193.530, P <0.05) in the experimental group were significantly higher than the baseline levels after phacoemulsification. In the clinical study, NRS scores of second eye surgery were higher than those of the first eye(P =0.0025) and 19 (63.3%) patients reported more pain during the second eye surgery. TNF- and IL-1 concentrations in AH of the second eye were significantly higher than those of the first eye (F =123.60, P <0.05; F =59.60, P <0.05). In conclusion, within 1 month after the first eye phacoemulsification, higher pain sensitivity (hyperalgesia) exists in the second eye, which may be related to dynamic changes in TNF-, IL-1 levels in AH or whole blood.
本研究旨在探讨第一只眼白内障手术后第二只眼术中痛觉过敏与眼房水（AH）和全血中肿瘤坏死因子（TNF）-和白细胞介素（IL）-1 水平的动态变化之间的相关性。通过给予 0.3%左氧氟沙星建立单侧白内障超声乳化术兔模型。实验组（n=25）非手术眼和空白对照组（n=15）第二眼获得全血和 AH 样本，并在术后（术后 1、3、7、14 和 21 天）检查角膜敏感性。测量 AH 中的 TNF-和 IL-1 水平以及全血中的 TNF-mRNA 和 IL-1mRNA 水平。在一项临床研究中，将在 1 个月内接受双侧白内障超声乳化术的 30 例患者根据手术间隔时间分为六组（1、3、7、10、14 和 21 天）。测量手术开始时 AH 中的 TNF-和 IL-1 水平，并在手术后立即评估术中疼痛。实验组非手术眼和全血中的角膜敏感性（F=244.910，P<0.05）、TNF-和 IL-1 水平（F=184.200、82.900，P<0.05）均明显高于白内障超声乳化术后的基线水平。在临床研究中，第二只眼手术的 NRS 评分高于第一只眼（P=0.0025），19 例（63.3%）患者报告第二只眼手术期间疼痛更严重。第二只眼 AH 中的 TNF-和 IL-1 浓度明显高于第一只眼（F=123.60，P<0.05；F=59.60，P<0.05）。总之，在第一只眼白内障超声乳化术后 1 个月内，第二只眼存在更高的疼痛敏感性（痛觉过敏），这可能与 AH 或全血中 TNF-、IL-1 水平的动态变化有关。
