De Boeck Ilke, Wittouck Stijn, Martens Katleen, Spacova Irina, Cauwenberghs Eline, Allonsius Camille Nina, Jörissen Jennifer, Wuyts Sander, Van Beeck Wannes, Dillen Jelle, Bron Peter A, Steelant Brecht, Hellings Peter W, Vanderveken Olivier M, Lebeer Sarah
Department of Bioscience Engineering, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerp, Belgium.
KU Leuven Department of Microbiology, Immunology and Transplantation, Allergy and Clinical Immunology Research Unit, Herestraat 49, 3000 Leuven, Belgium.
iScience. 2021 Aug 13;24(9):102978. doi: 10.1016/j.isci.2021.102978. eCollection 2021 Sep 24.
Comparing the nasal microbiome of healthy individuals and chronic rhinosinusitis (CRS) patients revealed as a species clearly associated with nasal health, although isolates obtained from healthy individuals are scarce. In this study, we explored the properties of this understudied lactic acid bacterium by integrating comparative genomics, habitat mining, cultivation, and functional characterization of interaction capacities. Mining 10.000 samples from the Earth Microbiome Project of 17 habitat types revealed that is mainly associated with the human nasal cavity. AMBR11 isolated from the nose of a healthy individual exerted antimicrobial activity against , decreased proinflammatory cytokine production in airway epithelial cells, and larvae mortality induced by this important nasal pathobiont. Furthermore, the strain protected the nasal barrier function in a mouse model using interleukin-4 as disruptive cytokine. Hence, AMBR11 is a mutualist with high potential as topical live biotherapeutic product.
比较健康个体和慢性鼻-鼻窦炎（CRS）患者的鼻腔微生物群发现， 是一种与鼻腔健康明显相关的物种，尽管从健康个体分离出的菌株很少。在本研究中，我们通过整合比较基因组学、栖息地挖掘、培养以及相互作用能力的功能表征，探索了这种研究较少的乳酸菌的特性。从地球微生物组计划的17种栖息地类型中挖掘10000个样本发现， 主要与人类鼻腔相关。从一名健康个体的鼻子中分离出的AMBR11对 具有抗菌活性，可降低气道上皮细胞中促炎细胞因子的产生，并降低由这种重要的鼻腔致病共生菌诱导的 幼虫死亡率。此外，在以白细胞介素-4作为破坏细胞因子的小鼠模型中，该菌株保护了鼻腔屏障功能。因此，AMBR11是一种具有高潜力的共生菌，可作为局部活体生物治疗产品。
