Belayneh Yaschilal Muche, Amare Gedefaw Getnet, Meharie Birhanu Geta, Kifle Zemene Demelash
Department of Pharmacy, College of medicine and Health Sciences, Wollo University, P.O. Box 1145, Dessie, Ethiopia.
Department of Pharmacology, School of Pharmacy, College of Medicine and Health Sciences, University of Gondar, Gondar, Ethiopia.
Metabol Open. 2021 Aug 24;11:100119. doi: 10.1016/j.metop.2021.100119. eCollection 2021 Sep.
The aim of this study was to evaluate the antiulcer activity of hydromethanol extracts of L. (Solanaceae) leaves and roots in mice.
The antiulcerogenic activity of the plant extracts were evaluated using Pylorus ligation and ethanol induced gastric ulcers in fasted mice. Data were analyzed using one way ANOVA, and P-values <0.05 were considered statistically significant.
RESULTPYLORUS LIGATION-INDUCED ULCER: Single dose and repeated daily dose administration of the leaf and root extracts for 10 days didn't significantly (P > 0.05) affect pH, total acidity and volume of gastric secretion. Single dose of both extracts significantly reduced ulcer score (P = 0.036) and ulcer index (leaf, P = 0.037; root, P = 0.041) at the dose of 400 mg/kg. Similarly, significant reduction in ulcer score was observed after repeated daily treatment with 200 mg/kg (P = 0.030) and 400 mg/kg (P = 0.005) of the leaf extract and 400 mg/kg (P = 0.005) of the root extract. In addition, repeated administration of 400 mg/kg of the leaf (P = 0.004) and root (P = 0.005) extracts significantly reduced ulcer index.
ETHANOL-INDUCED ULCER: Single dose of both extracts significantly reduced ulcer score at the dose of 200 mg/kg (leaf, P = 0.017; root, P = 0.036) and 400 mg/kg (leaf, P = 0.001; root, P = 0.001). Similarly, 200 mg/kg (leaf, P = 0.002; root, P = 0.018) and 400 mg/kg (leaf, P = 0.001; root, P = 0.001) of the extracts significantly reduced ulcer index after single dose treatment. Repeated daily treatment with leaf and root extracts for ten days caused a significant (P = 0.037, 0.001 and 0.001 for 100, 200 and 400 mg/kg leaf extract; P = 0.026, 0.018 and 0.001 for 100, 200 and 400 mg/kg root extract, respectively) reduction in ulcer score. In addition, both extracts significantly (P = 0.041, 0.004 and 0.000 for 100, 200 and 400 mg/kg leaf extract; P = 0.038, 0.008 and 0.000 for 100, 200 and 400 mg/kg root extract, respectively) reduced ulcer index after 10 days of treatment.
This study has revealed hydromethanol extracts of leaves and roots have antiulcerogenic activity using models. The antiulcer activity of the plant is not related to acid anti-secretory action, suggesting the plant may have cytoprotective effect on the gastric mucosa.
本研究旨在评估茄科某植物叶和根的水甲醇提取物对小鼠的抗溃疡活性。
采用幽门结扎法和乙醇诱导禁食小鼠胃溃疡，评估植物提取物的抗溃疡活性。数据采用单因素方差分析，P值<0.05被认为具有统计学意义。
结果
叶和根提取物单剂量及连续10天每日重复给药对胃液pH值、总酸度和分泌量均无显著影响（P>0.05）。两种提取物单剂量400mg/kg时均显著降低溃疡评分（P=0.036）和溃疡指数（叶，P=0.037；根，P=0.041）。同样，叶提取物200mg/kg（P=0.030）和400mg/kg（P=0.005）以及根提取物400mg/kg（P=0.005）连续每日治疗后，溃疡评分显著降低。此外，叶提取物400mg/kg（P=0.004）和根提取物400mg/kg（P=0.005）重复给药显著降低溃疡指数。
两种提取物单剂量200mg/kg（叶，P=0.017；根，P=0.036）和400mg/kg（叶，P=0.001；根，P=0.001）时均显著降低溃疡评分。同样，单剂量治疗后，提取物200mg/kg（叶，P=0.002；根，P=0.018）和400mg/kg（叶，P=0.001；根，P=0.001）显著降低溃疡指数。叶和根提取物连续10天每日治疗导致溃疡评分显著降低（叶提取物100、200和400mg/kg分别为P=0.037、0.001和0.001；根提取物100、200和400mg/kg分别为P=0.026、0.018和0.001）。此外，治疗10天后，两种提取物均显著降低溃疡指数（叶提取物100、200和400mg/kg分别为P=0.041、0.004和0.000；根提取物100、200和400mg/kg分别为P=0.038、0.008和0.000）。
本研究表明，该植物叶和根的水甲醇提取物在两种模型中均具有抗溃疡活性。该植物的抗溃疡活性与抗酸分泌作用无关，提示该植物可能对胃黏膜具有细胞保护作用。
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