Haile Firnus, G/Medhin Markeshaw Tiruneh, Kifle Zemene Demelash, Dejenie Tadesse Asmamaw, Berhane Nega
Institute of Biotechnology, University of Gondar, Gondar, Ethiopia.
Department of Medical Biochemistry, School of Medicine, College of Medicine and Health Sciences, University of Gondar, Gondar, Ethiopia.
Metabol Open. 2022 Sep 15;16:100210. doi: 10.1016/j.metop.2022.100210. eCollection 2022 Dec.
Medicinal plants have been used as traditional treatments for various human diseases for many years and they are still widely practiced throughout the world. Due to the long history of the practice, medicinal plants have become an integral part of the Ethiopian culture. This study aimed to evaluate the antibacterial activities of Hiern and Fresen leaf extracts and their synergistic effect against some selected human pathogenic bacteria.
Ethanol, methanol, and n-hexane crude extracts of and a mixture of the two-plant respective of each solvent were evaluated against tested pathogenic bacteria using the agar well diffusion method; the inhibition zones were recorded in millimeters. Gentamycin was used as a positive control, while dimethyl sulfoxide served as a negative control. The minimum inhibitory concentration of the plant extracts against test bacteria was evaluated using two-fold broth dilution methods and then Minimum bactericidal concentration was determined by sub-culturing the test dilutions from minimum inhibitory concentration tubes onto fresh Muller Hinton Agar plates incubated at 37 °C for 24 h.
Maximum antibacterial inhibition zone was observed on methanol extracts of synergism against (ATCC 1333) (31.00 ± 1.73 mm) while, a minimum inhibition zone was observed on methanol extract of , against (ATCC 35218) (5.67 ± 0.57). Minimum inhibitory concentration and minimum bactericidal concentration values of the crude extracts of , , and their mixture lies between (3.125%-12.5%) and (6.25%-25%) respectively. The data were analyzed using the SPSS software package version 20 for windows.
The present study revealed that ethanol and methanol extracts of and possess significant inhibitory effects against tested pathogens and the antibacterial activity of both plants leaf extracts was greater than the activity of currently used antibiotics (Gentamycin) against some selected organisms.
药用植物多年来一直被用作治疗各种人类疾病的传统疗法，并且在全世界仍被广泛应用。由于这种应用历史悠久，药用植物已成为埃塞俄比亚文化中不可或缺的一部分。本研究旨在评估希恩叶提取物和弗雷森叶提取物的抗菌活性及其对某些选定人类病原菌的协同作用。
使用琼脂孔扩散法，对希恩和弗雷森的乙醇、甲醇和正己烷粗提物以及每种溶剂中两种植物的混合物进行测试，以对抗受试病原菌；抑菌圈以毫米为单位记录。庆大霉素用作阳性对照，而二甲基亚砜用作阴性对照。采用两倍肉汤稀释法评估植物提取物对受试细菌的最低抑菌浓度，然后通过将最低抑菌浓度管中的受试稀释液转接至新鲜的穆勒-欣顿琼脂平板上，于37℃培养24小时来确定最低杀菌浓度。
在针对金黄色葡萄球菌（ATCC 1333）的甲醇协同提取物上观察到最大抑菌圈（31.00±1.73毫米），而在针对肺炎克雷伯菌（ATCC 35218）的甲醇提取物上观察到最小抑菌圈（5.67±0.57）。希恩、弗雷森及其混合物的粗提物的最低抑菌浓度和最低杀菌浓度值分别在（3.125%-12.5%）和（6.25%-25%）之间。使用适用于Windows的SPSS软件包版本20对数据进行分析。
本研究表明，希恩和弗雷森的乙醇和甲醇提取物对受试病原菌具有显著抑制作用，并且两种植物叶提取物的抗菌活性均大于目前使用的抗生素（庆大霉素）对某些选定生物体的活性。
