Cancer Program, Monash Biomedicine Discovery Institute and Department of Biochemistry and Molecular Biology, Monash University, Melbourne, VIC 3800, Australia.
STAR Protoc. 2021 Aug 24;2(3):100765. doi: 10.1016/j.xpro.2021.100765. eCollection 2021 Sep 17.
3D cultures of mammary epithelial cells purified from murine models provide a unique resource to study genetically defined breast cancer and response to targeted therapies. Here, we describe step-by-step experimental procedures for the successful establishment of murine mammary organoid lines isolated from mammary glands or mammary tumors driven by mutations in components of the PI3K pathway. These detailed protocols also include procedures to perform assays that can be adopted to screen response to drug treatments and to inform better therapies. For details on potential applications and use of this protocol, please refer to Yip et al. (2020).
从鼠模型中纯化的乳腺上皮细胞 3D 培养物为研究遗传定义的乳腺癌和对靶向治疗的反应提供了独特的资源。在这里，我们描述了从乳腺或由 PI3K 通路成分突变驱动的乳腺肿瘤中分离的成功建立鼠类乳腺类器官系的逐步实验步骤。这些详细的方案还包括进行可以采用的测定的程序，以筛选对药物治疗的反应，并提供更好的治疗方法。有关该方案的潜在应用和使用的详细信息，请参阅 Yip 等人。（2020 年）。
