结扎小鼠左前降支冠状动脉以建立心肌梗死模型的手术方案。

Surgical protocol for permanent ligation of the left anterior descending coronary artery in mice to generate a model of myocardial infarction.

机构信息

Department of Physiological Chemistry and Metabolism, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.

Department of Pathology and Matrix Biology, Graduate School of Medicine, Mie University Graduate School of Medicine, 2-174 Edobashi Tsu, Mie 514-8507, Japan.

出版信息

STAR Protoc. 2021 Aug 26;2(3):100775. doi: 10.1016/j.xpro.2021.100775. eCollection 2021 Sep 17.

DOI:10.1016/j.xpro.2021.100775

PMID:34485941

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8403767/

Abstract

Myocardial infarction (MI) is one of the most common causes of death worldwide. Animal models for MI are useful for studying the pathophysiology and developing therapies. Here, we describe a surgical protocol for permanent ligation of the left anterior descending coronary artery in mice, which mimics human acute coronary syndrome. This protocol includes descriptive step-by-step surgical procedures and high-quality surgical videos, which are useful for performing stable and highly reproducible operations. For complete details on the use and execution of this protocol, please refer to Maruyama et al. (2021).

摘要

心肌梗死（MI）是全球范围内最常见的死亡原因之一。MI 的动物模型对于研究病理生理学和开发治疗方法非常有用。在这里，我们描述了一种在小鼠中永久性结扎左前降支冠状动脉的手术方案，该方案模拟了人类急性冠状动脉综合征。本方案包括描述性的逐步手术步骤和高质量的手术视频，对于进行稳定且高度可重复的操作非常有用。有关该方案的使用和执行的完整详细信息，请参阅 Maruyama 等人。（2021 年）。