Department of Pharmacy, Southwest Hospital affiliated to the Army Medical University.
Department of Nephrology, Xinqiao Hospital affiliated to the Army Medical University, Chongqing, P.R. China.
Anticancer Drugs. 2022 Jan 1;33(1):e711-e719. doi: 10.1097/CAD.0000000000001246.
In our paper, the effects of As4S4 treatments on the growth and migration of gastric cancer (GC) cells were explored, and the potential underlying molecular mechanisms were also identified. Cell viability was evaluated by cell counting kit 8 assay. The expression of Ki-67 was examined using immunofluorescence staining. Cell apoptosis was assessed by flow cytometry. The migratory and invasion abilities of cells were determined using Transwell assay. The mRNA and protein levels of related gene were examined by RT-qPCR and western blotting, respectively. CircRNAs chip was performed to identify the differentiated expression of circRNAs in GC cells following the treatment with As4S4. Our results revealed that the proliferation, migration and invasion of GC cells were remarkably suppressed by the treatment with As4S4, while cell apoptosis was promoted. Furthermore, circRNA_ASAP2 was a novel target of As4S4 in GC, and it is involved in As4S4-modulated biological behavior alterations in GC cells. In addition, the activities of the Wnt/β-catenin signaling in GC cells were affected by the overexpression circRNA_ASAP2 and the treatment with As4S4. Moreover, the behavior changes in GC cells caused by the knockdown of circRNA_ASAP2 were reversed by the treatment with Wnt agonist SKL2001. In summary, As4S4 could function as an antitumor agent in GC through regulating the circRNA_ASAP2/Wnt/β-catenin pathway, which in turn influences the growth and metastasis of GC cells.
在我们的论文中，探讨了 As4S4 处理对胃癌（GC）细胞生长和迁移的影响，并确定了潜在的分子机制。通过细胞计数试剂盒 8 测定细胞活力。使用免疫荧光染色检测 Ki-67 的表达。通过流式细胞术评估细胞凋亡。通过 Transwell 测定评估细胞的迁移和侵袭能力。通过 RT-qPCR 和 Western blot 分别检测相关基因的 mRNA 和蛋白水平。通过 circRNA 芯片鉴定了 As4S4 处理后 GC 细胞中 circRNAs 的差异表达。我们的研究结果表明，As4S4 处理可显著抑制 GC 细胞的增殖、迁移和侵袭，同时促进细胞凋亡。此外，circRNA_ASAP2 是 GC 中 As4S4 的一个新靶点，它参与了 As4S4 调节的 GC 细胞生物学行为改变。此外，circRNA_ASAP2 的过表达和 As4S4 的处理影响了 GC 细胞中的 Wnt/β-catenin 信号通路活性。此外，circRNA_ASAP2 敲低引起的 GC 细胞行为变化可通过 Wnt 激动剂 SKL2001 的处理而逆转。总之，As4S4 可通过调节 circRNA_ASAP2/Wnt/β-catenin 通路在 GC 中发挥抗肿瘤作用，从而影响 GC 细胞的生长和转移。
