College of Life Science, Shanxi University, Taiyuan, China.
Shanxi Key Laboratory for Research and Development of Regional Plants, Shanxi University, Taiyuan, China.
Biotechnol Appl Biochem. 2022 Aug;69(4):1757-1764. doi: 10.1002/bab.2248. Epub 2021 Sep 14.
Rosmarinic acid (RosA) is a phenolic acid compound extracted from perilla. In this experiment, the Oxford cup method was used to verify the antibacterial activity of PerillaRosA against Escherichia coli, Staphylococcus aureus, Salmonella, and Bacillus subtilis. By polyacrylamide gel electrophoresis, the effect of RosA on bacterial nucleic acid and bacterial Na /K -ATP-ase activity, and scanning electron microscope to exploration of its antibacterial mechanism preliminarily. The results showed that RosA had antibacterial properties against all four bacteria. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of E. coli were 0.8 and 0.9 mg/ml, respectively. The MIC and MBC of Salmonella were 0.9 and 1.0 mg/ml, respectively. The MIC and MBC of S. aureus and B. subtilis were both 1.0 and 1.1 mg/ml. RosA has the bacteriostasis function, which can destroy bacterial cells and cell proteins and inhibit the activity of Na /K -ATP-ase in cells.
迷迭香酸（RosA）是一种从紫苏中提取的酚酸类化合物。本实验采用牛津杯法验证了紫苏迷迭香酸（PerillaRosA）对大肠杆菌、金黄色葡萄球菌、沙门氏菌和枯草芽孢杆菌的抗菌活性。通过聚丙烯酰胺凝胶电泳研究了 RosA 对细菌核酸和细菌 Na / K -ATP 酶活性的影响，并通过扫描电子显微镜初步探讨了其抗菌机制。结果表明，RosA 对这四种细菌均具有抗菌作用。大肠杆菌的最小抑菌浓度（MIC）和最小杀菌浓度（MBC）分别为 0.8 和 0.9 mg/ml。沙门氏菌的 MIC 和 MBC 分别为 0.9 和 1.0 mg/ml。金黄色葡萄球菌和枯草芽孢杆菌的 MIC 和 MBC 均为 1.0 和 1.1 mg/ml。RosA 具有抑菌作用，能破坏细菌细胞和细胞蛋白，并抑制细胞内 Na / K -ATP 酶的活性。
