Chen Jiankang, Xu Jiayi, Wang Ping, Wang Yihan, Wang Yumeng, Lian Junmei, Yan Yan, Cheng Lin, Wang Yingping, Di Peng
State Local Joint Engineering Research Center of Ginseng Breeding and Application, College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China.
Int J Mol Sci. 2024 Dec 22;25(24):13717. doi: 10.3390/ijms252413717.
(L.) Britt. is a traditional medicinal and culinary plant with a long history of cultivation and significant potential for broader utilization. The basic helix-loop-helix () gene family is essential for regulating plant growth, development, stress responses, and secondary metabolism. However, the gene family in has not yet been characterized. In this study, a total of 205 genes were identified in through genome mining and analysis. Phylogenetic analysis classified these genes into 23 distinct subfamilies. Promoter analysis revealed an enrichment of cis-acting elements linked to plant hormone signaling and stress responses, suggesting their potential regulatory roles in development, growth, and stress adaptation. Expression profiling based on publicly available RNA-seq data demonstrated tissue-specific expression patterns of genes in roots, stems, and leaves. Four genes (, , , and ) showed significant responses to methyl jasmonate (MeJA) induction. Yeast one-hybrid assays confirmed that these PfbHLH proteins could bind to the cis-acting G-box (CACGTG) element. This study offers new perspectives on the evolution, regulatory mechanisms, and functional roles of the gene family in . The findings deepen our understanding of the functional diversity within this gene family and establish a foundation for genetic enhancement and the biosynthesis of medicinal compounds in the species.
(L.) Britt.是一种传统的药用和食用植物，有着悠久的种植历史和广泛利用的巨大潜力。基本螺旋-环-螺旋（bHLH）基因家族对于调控植物生长、发育、应激反应和次生代谢至关重要。然而，该植物中的bHLH基因家族尚未得到表征。在本研究中，通过基因组挖掘和分析，在该植物中总共鉴定出205个bHLH基因。系统发育分析将这些bHLH基因分为23个不同的亚家族。启动子分析揭示了与植物激素信号传导和应激反应相关的顺式作用元件的富集，表明它们在发育、生长和应激适应中的潜在调控作用。基于公开可用的RNA测序数据的表达谱分析表明bHLH基因在根、茎和叶中具有组织特异性表达模式。四个bHLH基因（、、和）对茉莉酸甲酯（MeJA）诱导有显著反应。酵母单杂交试验证实这些PfbHLH蛋白可以与顺式作用G盒（CACGTG）元件结合。本研究为该植物中bHLH基因家族的进化、调控机制和功能作用提供了新的视角。这些发现加深了我们对该基因家族内功能多样性的理解，并为该物种的遗传改良和药用化合物的生物合成奠定了基础。
