Instituto de Biotecnologia, Unidad Universitaria de Secuenciacion Masiva Y Bioinformatica, Universidad Nacional Autonoma de Mexico, Cuernavaca, Mexico.
LaNaBio, Instituto de Biologia, UNAM, Mexico City, Mexico.
BMC Res Notes. 2021 Sep 7;14(1):333. doi: 10.1186/s13104-021-05746-x.
Cenotes are flooded caves in Mexico's Yucatan peninsula. Many cenotes are interconnected in an underground network of pools and streams forming a vast belowground aquifer across most of the peninsula. Many plants in the peninsula grow roots that reach the cenotes water and live submerged in conditions similar to hydroponics. Our objective was to study the microbial community associated with these submerged roots of the Sac Actun cenote. We accomplished this objective by profiling the root prokaryotic community using 16S rRNA gene amplification and sequencing.
We identified plant species by DNA barcoding the total genomic DNA of each root. We found a distinctive composition of the root and water bacterial and archaeal communities. Prokaryotic diversity was higher in all plant roots than in the surrounding freshwater, suggesting that plants in the cenotes may attract and select microorganisms from soil and freshwater, and may also harbor vertically transmitted lineages. The reported data are of interest for studies targeting biodiversity in general and root-microbial ecological interactions specifically.
天坑是墨西哥尤卡坦半岛上被水淹没的洞穴。许多天坑相互连通，形成了一个地下池塘和溪流网络，构成了半岛上大部分地区广阔的地下含水层。半岛上的许多植物生长出延伸到天坑水中的根系，并在类似于水培的条件下生活在水下。我们的目的是研究与 Sac Actun 天坑这些水下根系相关的微生物群落。我们通过使用 16S rRNA 基因扩增和测序来描绘根系原核生物群落，从而实现了这一目标。
我们通过对每个根系的总基因组 DNA 进行 DNA 条形码鉴定，确定了植物物种。我们发现了根系和水细菌和古菌群落的独特组成。所有植物根系中的原核生物多样性都高于周围的淡水，这表明天坑中的植物可能从土壤和淡水中吸引和选择微生物，并且可能还拥有垂直传播的谱系。所报告的数据对于针对生物多样性和根系微生物生态相互作用的研究具有重要意义。
