College of Life Sciences, Huaibei Normal University, Huaibei 235000, China.
ACS Appl Mater Interfaces. 2021 Sep 22;13(37):44329-44338. doi: 10.1021/acsami.1c11815. Epub 2021 Sep 8.
In the present study, a magnetic mimic multi-enzyme system was developed by encapsulating the aryloxyphenoxypropionate (AOPP) herbicide hydrolase QpeH and alcohol oxidase (AOx) in zeolitic imidazolate framework (ZIF-8) nanocrystals with magnetic FeO nanoparticles (MNPs) to detect AOPP herbicides. The structural, protein loading capacity and loading ratio, porosity, and magnetic properties of QpeH/AOx@mZIF-8 were characterized by scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, thermogravimetric analysis, nitrogen sorption, and vibrating sample magnetometry. An AOPP herbicide colorimetric biosensor made with QpeH/AOx@mZIF-8 had the highest sensitivity toward quizalofop-P-ethyl (QpE) with a limit of detection of 8.2 μM. This system was suitable to detect two other AOPP herbicides, including fenoxaprop-P-ethyl (FpE) and haloxyfop-P-methyl (HpE). The practical application of the biosensor was verified through quantitative analysis of QpE residues in industrial wastewater and field soils. Furthermore, QpeH/AOx@mZIF-8 exhibited excellent long-term storage stability (at least 50 days), easy separation by magnet, and reusability (at least 10 cycles), supporting its promising role in simple and low-cost detection of AOPP herbicides in real environmental samples.
在本研究中，通过将芳氧苯氧丙酸酯（AOPP）除草剂水解酶 QpeH 和醇氧化酶（AOx）封装在沸石咪唑酯骨架（ZIF-8）纳米晶体中，并与磁性 FeO 纳米颗粒（MNPs）结合，开发了一种磁性模拟多酶系统，用于检测 AOPP 除草剂。通过扫描电子显微镜、X 射线衍射、傅里叶变换红外光谱、热重分析、氮气吸附和振动样品磁强计对 QpeH/AOx@mZIF-8 的结构、蛋白载量和载量比、孔隙率和磁性进行了表征。使用 QpeH/AOx@mZIF-8 制备的 AOPP 除草剂比色生物传感器对 quizalofop-P-ethyl（QpE）具有最高的灵敏度，检测限为 8.2 μM。该系统适用于检测另外两种 AOPP 除草剂，包括 fenoxaprop-P-ethyl（FpE）和 haloxyfop-P-methyl（HpE）。通过对工业废水中和田间土壤中 QpE 残留的定量分析，验证了该生物传感器的实际应用。此外，QpeH/AOx@mZIF-8 表现出优异的长期储存稳定性（至少 50 天）、易于通过磁铁分离和可重复使用性（至少 10 次循环），支持其在实际环境样品中简单、低成本检测 AOPP 除草剂方面的应用前景。
