The University of Queensland Diamantina Institute, 37 Kent Street, Woolloongabba, Brisbane, 4102, Australia.
Cancer and Ageing Research Program, School of Biomedical Sciences, Queensland University of Technology, 37 Kent Street, Woolloongabba, Brisbane, 4102, Australia.
Angiogenesis. 2022 Feb;25(1):15-33. doi: 10.1007/s10456-021-09817-2. Epub 2021 Sep 9.
The cardiovascular system is composed around the central function of the endothelium that lines the inner surfaces of its vessels. In recent years, the existence of a progenitor population within the endothelium has been validated through the study of endothelial colony-forming cells (ECFCs) in human peripheral blood and certain vascular beds. However, our knowledge on endothelial populations in vivo that can give rise to ECFCs in culture has been limited. In this review we report and analyse recent attempts at describing progenitor populations in vivo from murine studies that reflect the self-renewal and stemness capacity observed in ECFCs. We pinpoint seminal discoveries within the field, which have phenotypically defined, and functionally scrutinised these endothelial progenitors. Furthermore, we review recent publications utilising single-cell sequencing technologies to better understand the endothelium in homeostasis and pathology.
心血管系统的组成围绕着内皮细胞的核心功能展开，内皮细胞排列在其血管的内表面。近年来，通过对人外周血和某些血管床中的内皮集落形成细胞（endothelial colony-forming cells，ECFCs）的研究，已经验证了内皮细胞中存在祖细胞群体。然而，我们对体内能够在培养中产生 ECFC 的内皮细胞群体的了解一直很有限。在这篇综述中，我们报告并分析了最近从反映 ECFC 中观察到的自我更新和干细胞特性的小鼠研究中描述体内祖细胞群体的尝试。我们指出了该领域的重要发现，这些发现已经从表型上定义并从功能上仔细研究了这些内皮祖细胞。此外，我们还回顾了最近利用单细胞测序技术来更好地理解内稳态和病理学中的内皮细胞的出版物。
