Benali Taoufiq, Lemhadri Ahmed, Harboul Kaoutar, Chtibi Houda, Khabbach Abdelmajid, Jadouali Si Mohamed, Quesada-Romero Luisa, Louahlia Said, Hammani Khalil, Ghaleb Adib, Lee Learn-Han, Bouyahya Abdelhakim, Rusu Marius Emil, Akhazzane Mohamed
Environment and Health Team, Polydisciplinary Faculty of Safi, Cadi Ayyad University, Marrakech 46030, Morocco.
Laboratory of Natural Resources and Environment, Polydisciplinary Faculty of Taza, Sidi Mohamed Ben Abdellah University of Fez, B.P. 1223 Taza-Gare, Taza 30050, Morocco.
Plants (Basel). 2023 Mar 22;12(6):1413. doi: 10.3390/plants12061413.
The aim of this study was the determination of the chemical compounds of essential oil from Aknol (LSEO), Khenifra (LSEO), and Beni Mellal (LSEO), and in vitro investigation of their antibacterial, anticandidal, and antioxidant effects, and in silico anti-SARS-CoV-2 activity. The chemical profile of LSEO was determined using GC-MS-MS analysis, the results of which showed a qualitative and quantitative variation in the chemical composition of volatile compounds including L-fenchone, cubebol, camphor, bornyl acetate, and τ-muurolol; indicating that the biosynthesis of essential oils of (LSEO) varied depending on the site of growth. The antioxidant activity was evaluated using the ABTS and FRAP methods, our results showed that this tested oil is endowed with an ABTS inhibitory effect and an important reducing power which varies between 4.82 ± 1.52 and 15.73 ± 3.26 mg EAA/g extract. The results of antibacterial activity of LSEO, LSEO and LSEO, tested against Gram-positive and Gram-negative bacteria, revealed that (20.66 ± 1.15-25 ± 4.35 mm), (18.66 ± 1.15-18.66 ± 1.15 mm), and (13.33 ± 1.15-19 ± 1.00 mm) are the most susceptible strains to LSEO, LSEO and LSEO of which LSEO exhibits bactericidal effect against . furthermore The LSEO exhibited varying degrees of anticandidal activity with an inhibition zones of 25.33 ± 0.5, 22.66 ± 2.51, and 19 ± 1 mm for LSEO, LSEO, and LSEO, respectively. Additionally, the in silico molecular docking process, performed using Chimera Vina and Surflex-Dock programs, indicated that LSEO could inhibit SARS-CoV-2. These important biological properties of LSEO qualify this plant as an interesting source of natural bioactive compounds with medicinal actions.
本研究的目的是测定阿克诺尔（LSEO）、凯尼弗拉（LSEO）和贝尼迈勒（LSEO）香精油的化学成分，并对其抗菌、抗念珠菌和抗氧化作用进行体外研究，以及进行计算机模拟抗SARS-CoV-2活性研究。使用气相色谱-质谱-质谱联用（GC-MS-MS）分析确定了LSEO的化学特征，结果显示挥发性化合物的化学成分在定性和定量上存在差异，包括L-葑酮、库贝醇、樟脑、乙酸龙脑酯和τ-依兰油醇；这表明（LSEO）香精油的生物合成因生长地点而异。使用ABTS和FRAP方法评估抗氧化活性，我们的结果表明，该受试油具有ABTS抑制作用和重要的还原能力，其变化范围在4.82±1.52至15.73±3.26 mg EAA/g提取物之间。对革兰氏阳性菌和革兰氏阴性菌进行测试的LSEO、LSEO和LSEO的抗菌活性结果显示，（20.66±1.15 - 25±4.35 mm）、（18.66±1.15 - 18.66±1.15 mm）和（13.33±1.15 - 19±1.00 mm）是对LSEO、LSEO和LSEO最敏感的菌株，其中LSEO对表现出杀菌作用。此外，LSEO表现出不同程度的抗念珠菌活性，LSEO、LSEO和LSEO的抑菌圈分别为25.33±0.5、22.66±2.51和19±1 mm。此外，使用Chimera Vina和Surflex-Dock程序进行的计算机模拟分子对接过程表明，LSEO可以抑制SARS-CoV-2。LSEO的这些重要生物学特性使这种植物成为具有药用作用的天然生物活性化合物的有趣来源。
