Department of Drug Technology and Pharmaceutical Biotechnology, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland.
Microbiome Immunobiology Laboratory, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla 12, 53-114 Wroclaw, Poland.
Molecules. 2021 Sep 6;26(17):5404. doi: 10.3390/molecules26175404.
We previously described the biosynthesis, isolation, and immunosuppressive activity of the selenium-containing polysaccharide fraction isolated from the mycelial culture of . Structural studies have shown that the fraction was a protein-containing mixture of high molar mass polysaccharides α- and β-glucans. However, which of the components of the complex fraction is responsible for the immunosuppressive activity non-typical for polysaccharides of fungal origin has not been explained. In the current study, we defined four-polysaccharide components of the Se-containing polysaccharide fraction determined their primary structure and examined the effect on T- and B-cell proliferation. The isolated Se-polysaccharides, α-1,4-glucan (M 2.25 × 10 g/mol), unbranched β-1,6-d-glucan, unbranched β-1,3-d-glucan and β-1,3-branched β-1,6-d-glucan (M 1.10 × 10 g/mol), are not typical as components of the cell wall of . All are biologically active, but the inhibitory effect of the isolated polysaccharides on lymphocyte proliferation was weaker, though more selective than that of the crude fraction.
我们之前描述了从 的菌丝体培养物中分离出的含硒多糖部分的生物合成、分离和免疫抑制活性。结构研究表明，该部分是一种含有高摩尔质量多糖 α-和 β-葡聚糖的蛋白质混合物。然而，复杂部分的哪些成分负责非典型的真菌来源多糖的免疫抑制活性尚不清楚。在本研究中，我们确定了含硒多糖部分的四种多糖成分，确定了它们的一级结构，并研究了它们对 T 细胞和 B 细胞增殖的影响。分离得到的 Se 多糖，α-1,4-葡聚糖（M 2.25×10 g/mol）、无支链β-1,6-d-葡聚糖、无支链β-1,3-d-葡聚糖和β-1,3-支链β-1,6-d-葡聚糖（M 1.10×10 g/mol），作为 的细胞壁成分并不典型。所有这些都是具有生物活性的，但分离得到的多糖对淋巴细胞增殖的抑制作用较弱，尽管比粗提物更具选择性。
