Key Laboratory of Marine Drugs of Ministry of Education & Shandong Provincial Key Laboratory of Glycoscience and Glycotechnology, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China.
Laboratory for Marine Drugs and Bioproducts, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China.
Molecules. 2019 Apr 24;24(8):1610. doi: 10.3390/molecules24081610.
The purpose of this study is to develop a robust approach to obtain β glucans from and to characterize their structural and biological properties for sustainable utilization. The alkali extraction was optimized with an orthogonal experimental design, and a concise process for obtaining specific targeting polysaccharides from was developed in this study. After purification with a Q-Sepharose Fast Flow strong anion-exchange column, the monosaccharide composition, a methylation analysis, and NMR spectroscopy were employed for their structural characterizations. LeP-N2 was found to be composed of (1→6)-β-d-glucans with minor β-(1→3) glucosidic side chains. Atomic force microscopy (AFM) and high-performance gel permeation chromatography-refractive index-multi-angle laser light scattering (HPGPC-RI-MALLS) also revealed LeP-N2 exhibiting a compact unit in aqueous solution. This (1→6)-β-d-glucan was tested for antioxidant activities with IC at 157 μg/mL. Moreover, RAW 264.7 macrophage activation indicated that the release of nitric oxide (NO) and reactive oxygen species (ROS) was markedly increased with no cytotoxicity at a dose of 100 μg/mL. These findings suggest that the (1→6)-β-d-glucans obtained from could serve as potential agents in the fields of functional foods or medicine.
本研究旨在开发一种从 中获取β葡聚糖的稳健方法，并对其结构和生物学特性进行表征，以实现可持续利用。采用正交实验设计对碱提取进行了优化，本研究开发了一种从 中获得特定靶向多糖的简洁工艺。经 Q-Sepharose Fast Flow 强阴离子交换柱纯化后，采用单糖组成分析、甲基化分析和 NMR 光谱对其结构特征进行了研究。结果表明，LeP-N2 由（1→6）-β-d-葡聚糖组成，带有少量β-（1→3）葡萄糖苷侧链。原子力显微镜（AFM）和高效凝胶渗透色谱-折射率-多角度激光光散射（HPGPC-RI-MALLS）也表明，LeP-N2 在水溶液中呈现紧凑的单元。该（1→6）-β-d-葡聚糖的抗氧化活性测定 IC 为 157μg/mL。此外，RAW 264.7 巨噬细胞激活表明，在 100μg/mL 剂量下，一氧化氮（NO）和活性氧（ROS）的释放显著增加，且无细胞毒性。这些发现表明，从 中获得的（1→6）-β-d-葡聚糖可用作功能性食品或药物领域的潜在药物。
