Helmholz Heike, Adejube Blessing, Luthringer-Feyerabend Bérengère, Willumeit-Römer Regine
Helmholtz Zentrum Hereon, Institute of Metallic Biomaterials, Max Planck St., 21502 Geesthacht, Germany.
Faculty of Engineering, Cristian Albrecht University Kiel, Kaiserstraße 2, 24143 Kiel, Germany.
Materials (Basel). 2021 Sep 1;14(17):5005. doi: 10.3390/ma14175005.
Biomedical applications of magnesium (Mg) and its alloys are generally dependent on their degradation behavior in vivo. Despite its attractive properties, which make Mg suitable for orthopedic applications, the in vivo material-tissue (bone, blood, and lymph tissues) interaction is not yet fully understood. To investigate the influence of major serum proteins on the degradation, this study focused on fetuin, which is one of the major non-collagenous plasma proteins and which is essential for biomineralization. This study used a physiological setup to investigate the influence of fetuin on the degradation behavior of pure Mg in the presence of calcium (Ca). Extruded pure Mg samples were immersed under cell culture conditions in Hank's balanced salt solution (HBSS) under defined Ca regimes. The results showed a significant decrease in the degradation rate (DR) when both fetuin and Ca were present in an immersion medium as compared to media where they were not simultaneously present. A possible reason for this behavior was the forming of a dense, protein-degradation products protection barrier at the material surface. Furthermore, the limitation of freely available Ca might be a reason for a decreased degradation. The cultivation of primary osteoblasts (pOB) was possible at the fetuin-coated Mg-surface without additional serum supplementation.
镁（Mg）及其合金的生物医学应用通常取决于它们在体内的降解行为。尽管镁具有吸引人的特性，使其适用于骨科应用，但体内材料与组织（骨、血液和淋巴组织）之间的相互作用尚未完全了解。为了研究主要血清蛋白对降解的影响，本研究聚焦于胎球蛋白，它是主要的非胶原蛋白血浆蛋白之一，对生物矿化至关重要。本研究采用生理学设置来研究胎球蛋白在有钙（Ca）存在的情况下对纯镁降解行为的影响。将挤压的纯镁样品在细胞培养条件下，在规定的钙条件下浸泡于汉克斯平衡盐溶液（HBSS）中。结果表明，与不同时存在胎球蛋白和钙的培养基相比，当浸泡介质中同时存在胎球蛋白和钙时，降解速率（DR）显著降低。这种行为的一个可能原因是在材料表面形成了致密的蛋白质降解产物保护屏障。此外，游离钙的限制可能是降解减少的一个原因。在不额外补充血清的情况下，在胎球蛋白包被的镁表面可以培养原代成骨细胞（pOB）。
