Institute of Materials Research, Division for Metallic Biomaterials, Helmholtz-Zentrum Geesthacht (HZG), Max-Planck-Str. 1, 21502 Geesthacht, Germany.
Institute of Materials Research, Division for Metallic Biomaterials, Helmholtz-Zentrum Geesthacht (HZG), Max-Planck-Str. 1, 21502 Geesthacht, Germany.
Acta Biomater. 2020 Oct 15;116:426-437. doi: 10.1016/j.actbio.2020.08.043. Epub 2020 Sep 2.
Due to its degradability, magnesium holds potential for the application as a base material for local treatment systems. Particularly for the therapy of severe brain-related diseases, local approaches are advantageous. To confirm the suitability of magnesium as a material for neural implants, information on the interaction of brain cells with magnesium is essential. Initial steps of such an evaluation need to include not only cytocompatibility tests but also the analysis of the in vitro material degradation to predict in vivo material performance. Considering the sensitivity and functional importance of neural tissue, an in-depth understanding of the processes involved is of particular relevance. Here, we investigate the influence of four different brain cell types and fibroblasts on magnesium degradation in direct material contact. Our findings indicate cell type as well as cell density-dependent degradation behavior. Metabolic activity (lactate content) appears to be crucial for degradation promotion. Extracellular matrix composition, distribution, and matrix/cell ratios are analyzed to elucidate the cell-material interactions further. Statement of Significance Thanks to their degradability, magnesium (Mg)-based materials could be promising biomaterials for local ion or even drug delivery strategies for the treatment of severe brain-related diseases. To confirm the suitability of Mg as a neural implant material, information on the interaction of brain cells with Mg is essential. Initial steps of such an evaluation need to include cytocompatibility tests and the analysis of the in vitro material degradation to predict in vivo material performance. The present study provides data on the influence of different brain cell types on Mg degradation in direct material contact. Our findings indicate cell type and cell density-dependent degradation behavior, and elucidate the role of cell metabolites and extracellular matrix molecules in the underlying degradation mechanisms.
由于其可降解性，镁具有作为局部治疗系统基础材料的应用潜力。特别是对于严重的与大脑相关的疾病的治疗，局部方法是有利的。为了确认镁作为神经植入材料的适用性，关于脑细胞与镁相互作用的信息是必不可少的。这种评估的初始步骤不仅需要包括细胞相容性测试，还需要分析体外材料降解以预测体内材料性能。考虑到神经组织的敏感性和功能重要性，深入了解所涉及的过程尤为重要。在这里，我们研究了四种不同的脑细胞类型和成纤维细胞对直接材料接触中镁降解的影响。我们的研究结果表明，细胞类型和细胞密度依赖性的降解行为。代谢活性（乳酸含量）似乎对降解促进至关重要。进一步分析细胞外基质的组成、分布和基质/细胞比例，以阐明细胞-材料相互作用。
意义陈述
由于其可降解性，基于镁（Mg）的材料可能是治疗严重与大脑相关疾病的局部离子甚至药物输送策略的有前途的生物材料。为了确认镁作为神经植入材料的适用性，关于脑细胞与镁相互作用的信息是必不可少的。这种评估的初始步骤不仅需要包括细胞相容性测试，还需要分析体外材料降解以预测体内材料性能。本研究提供了关于不同脑细胞类型在直接材料接触中对 Mg 降解影响的数据。我们的研究结果表明，细胞类型和细胞密度依赖性的降解行为，并阐明了细胞代谢物和细胞外基质分子在潜在降解机制中的作用。
