Morikawa Daichi, Hawthorne Benjamin C, McCarthy Mary Beth R, Bellas Nicholas, Johnson Jeremiah D, Trudeau Maxwell T, Murphy Kyle V, Mancini Michael R, LeVasseur Matthew R, Cote Mark P, Mazzocca Augustus D
Department of Orthopaedic Surgery, University of Connecticut, Farmington, CT 06032, USA.
Department of Orthopaedic Surgery, Juntendo University Urayasu Hospital, Urayasu 279-0021, Japan.
J Clin Med. 2021 Sep 4;10(17):4006. doi: 10.3390/jcm10174006.
Unsatisfactory failure rates following rotator cuff (RC) repair have led orthopaedic surgeons to explore biological augmentation of the healing enthesis. The subacromial bursa (SB) contains abundant connective tissue progenitor cells (CTPs) that may aid in this process. The purpose of the study was to investigate the influence of patient demographics and tear characteristics on the number of colony-forming units (CFUs) and nucleated cell count (NCC) of SB-derived CTPs. In this study, we harvested SB tissue over the supraspinatus tendon and muscle in 19 patients during arthroscopic RC repair. NCC of each sample was analyzed on the day of the procedure. After 14 days, CFUs were evaluated under a microscope. Spearman's rank correlation coefficient was then used to determine the relationship between CFUs or NCC and patient demographics or tear characteristics. The study found no significant correlation between patient demographics and the number of CFUs or NCC of CTPs derived from the SB ( > 0.05). The study did significantly observe that increased tear size was negatively correlated with the number of CFUs ( < 0.05). These results indicated that increased tear size, but not patient demographics, may influence the viability of CTPs and should be considered when augmenting RCrepairs with SB.
肩袖（RC）修复术后不理想的失败率促使骨科医生探索对愈合的附着点进行生物增强。肩峰下囊（SB）含有丰富的结缔组织祖细胞（CTP），可能有助于这一过程。本研究的目的是调查患者人口统计学特征和撕裂特征对SB来源的CTP的集落形成单位（CFU）数量和成核细胞计数（NCC）的影响。在本研究中，我们在关节镜下进行RC修复时，从19例患者的冈上肌腱和肌肉上方采集了SB组织。在手术当天分析每个样本的NCC。14天后，在显微镜下评估CFU。然后使用Spearman等级相关系数来确定CFU或NCC与患者人口统计学特征或撕裂特征之间的关系。研究发现患者人口统计学特征与SB来源的CTP的CFU数量或NCC之间无显著相关性（>0.05）。该研究确实显著观察到，撕裂尺寸增大与CFU数量呈负相关（<0.05）。这些结果表明，撕裂尺寸增大而非患者人口统计学特征可能影响CTP的活力，在用SB增强RC修复时应予以考虑。
