用于分析tRNA基因DNA序列的IBM微型计算机程序。
IBM microcomputer programs that analyze DNA sequences for tRNA genes.
作者信息
Shortridge R D, Pirtle I L, Pirtle R M
机构信息
Department of Biochemistry, North Texas State University/Texas College of Osteopathic Medicine, Denton 76203.
出版信息
Comput Appl Biosci. 1986 Apr;2(1):13-7. doi: 10.1093/bioinformatics/2.1.13.
A set of four computer programs that search DNA sequence data files for transfer RNA genes have been written in IBM (Microsoft) BASIC for the IBM personal computer. These programs locate and plot predicted secondary structures of tRNA genes in the cloverleaf conformation. The set of programs are applicable to eukaryotic tRNA genes, including those containing intervening sequences, and to prokaryotic and mitochondrial tRNA genes. In addition, two of the programs search up to 150 residues downstream of tRNA gene sequences for possible eukaryotic RNA polymerase III termination sites comprised of at least four consecutive T residues. Molecular biologists studying a variety of gene sequence and flanking regions can use these programs to search for the additional presence of tRNA genes. Furthermore, investigators studying tRNA gene structure-to-function relationships would not need to do extensive restriction mapping to locate tRNA gene sequences within their cloned DNA fragments.
已经用适用于IBM个人计算机的IBM（微软）BASIC语言编写了一组四个计算机程序，用于在DNA序列数据文件中搜索转运RNA基因。这些程序定位并绘制呈三叶草构象的tRNA基因的预测二级结构。这组程序适用于真核tRNA基因，包括那些含有间隔序列的基因，以及原核和线粒体tRNA基因。此外，其中两个程序会在tRNA基因序列下游最多150个残基处搜索由至少四个连续T残基组成的可能的真核RNA聚合酶III终止位点。研究各种基因序列及其侧翼区域的分子生物学家可以使用这些程序来搜索tRNA基因的额外存在情况。此外，研究tRNA基因结构与功能关系的研究人员无需进行广泛的限制性图谱分析来在其克隆的DNA片段中定位tRNA基因序列。
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