Waldron C, Cox B S
Mol Gen Genet. 1978 Feb 16;159(2):223-5. doi: 10.1007/BF00270898.
We have examined the ribosomal proteins of strains of Saccharomyces cerevisiae which differ in the efficiency with which ochre nonsense mutations are suppressed. The strains in which ochre suppression is poor were [psi]- or carried antisuppressor mutations; those in which suppression was highly efficient were [psi]+ or carried allosuppressor mutations. The ribosomal proteins of these strains, as judged by two-dimensional polyacrylamide gel electrophoresis, were indistinguishable from those of wild-type.
我们检测了酿酒酵母菌株的核糖体蛋白,这些菌株在对赭石型无义突变的抑制效率上存在差异。赭石型抑制效果差的菌株为[psi]-型或携带反抑制突变;抑制效率高的菌株为[psi]+型或携带别种抑制突变。通过二维聚丙烯酰胺凝胶电泳判断,这些菌株的核糖体蛋白与野生型的核糖体蛋白没有区别。