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表达在乙型肝炎病毒相关肝细胞癌中的作用。

Involvement of Expression in Hepatitis B Virus-Related Hepatocellular Carcinoma.

作者信息

Zhu Shaoliang, Ye Hang, Xu Xiaojie, Huang Weiru, Peng Ziyu, Liao Yingyang, Peng Ningfu

机构信息

Department of Hepatobiliary Surgery, Guangxi Medical University Cancer Hospital, Nanning 530021, China.

Department of Clinical Nutrition, Guangxi Medical University Cancer Hospital, Nanning 530021, China.

出版信息

J Oncol. 2021 Aug 31;2021:8114327. doi: 10.1155/2021/8114327. eCollection 2021.

DOI:10.1155/2021/8114327
PMID:34512754
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8426092/
Abstract

OBJECTIVE

To investigate the expression of transient receptor potential (TRP) superfamily genes, especially in hepatitis B virus- (HBV-) related hepatocellular carcinoma (HCC).

METHODS

Three cancer samples of HBV-related HCC at phase IV and matched paracancerous liver tissues were included in the study. Total RNA was extracted, and differential expression of RNA was screened by high-throughput transcriptome sequencing. The expression of was detected by quantitative real-time PCR. The N6-adenosyl methylation RNA in MHCC97H, HepG2, and HL-7702 was enriched by coimmunoprecipitation with m6A antibody, and the relative level of N6-adenosyl methylation RNA in was detected.

RESULTS

The expression of TRP family genes in cancer tissues was higher than that in paracancerous liver tissues, including , , , and . Moreover, the expression level of in MHCC97H and HepG2 was also significantly higher than that in L02, a normal liver cell. The methylation level of N6-adenosine of was lower in HepG2 cells than that in L02 cells.

CONCLUSION

TRP superfamily genes, especially , were highly expressed in HBV-related HCC. could be a potential therapeutic target or diagnostic marker for HCC.

摘要

目的

研究瞬时受体电位（TRP）超家族基因的表达，尤其是在乙型肝炎病毒（HBV）相关肝细胞癌（HCC）中的表达。

方法

本研究纳入了3例IV期HBV相关HCC癌组织样本及配对的癌旁肝组织。提取总RNA，通过高通量转录组测序筛选RNA的差异表达。通过定量实时PCR检测[具体基因名称未给出]的表达。通过m6A抗体共免疫沉淀富集MHCC97H、HepG2和HL-7702中的N6-腺苷甲基化RNA，并检测[具体基因名称未给出]中N6-腺苷甲基化RNA的相对水平。

结果

癌组织中TRP家族基因的表达高于癌旁肝组织，包括[具体基因名称未给出]、[具体基因名称未给出]、[具体基因名称未给出]和[具体基因名称未给出]。此外，MHCC97H和HepG2中[具体基因名称未给出]的表达水平也显著高于正常肝细胞L02。HepG2细胞中[具体基因名称未给出]的N6-腺苷甲基化水平低于L02细胞。

结论

TRP超家族基因，尤其是[具体基因名称未给出]，在HBV相关HCC中高表达。[具体基因名称未给出]可能是HCC的潜在治疗靶点或诊断标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ffba/8426092/79ea610aa135/JO2021-8114327.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ffba/8426092/d1c17e97e7ed/JO2021-8114327.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ffba/8426092/e3926293d0e2/JO2021-8114327.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ffba/8426092/79ea610aa135/JO2021-8114327.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ffba/8426092/d1c17e97e7ed/JO2021-8114327.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ffba/8426092/e3926293d0e2/JO2021-8114327.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ffba/8426092/79ea610aa135/JO2021-8114327.003.jpg

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本文引用的文献

1
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Nat Cell Biol. 2019 May;21(5):542-551. doi: 10.1038/s41556-019-0311-8. Epub 2019 May 2.
2
Transient Receptor Potential (TRP) Channels.瞬时受体电位（TRP）通道
Subcell Biochem. 2018;87:141-165. doi: 10.1007/978-981-10-7757-9_6.
3
RNA N6-methyladenosine methyltransferase-like 3 promotes liver cancer progression through YTHDF2-dependent posttranscriptional silencing of SOCS2.RNA N6-甲基腺苷甲基转移酶样 3 通过 YTHDF2 依赖的 SOCS2 转录后沉默促进肝癌进展。
基于 TRP 家族的分子亚型和与 TRP 相关的长非编码 RNA 在胰腺腺癌中的预后评估。
BMC Gastroenterol. 2022 Nov 12;22(1):454. doi: 10.1186/s12876-022-02552-y.
4
Prognostic N6-methyladenosine (m6A)-related lncRNA patterns to aid therapy in pancreatic ductal adenocarcinoma.用于辅助胰腺导管腺癌治疗的预后性N6-甲基腺苷（m6A）相关长链非编码RNA模式
Front Genet. 2022 Sep 26;13:866340. doi: 10.3389/fgene.2022.866340. eCollection 2022.
5
A Comprehensive Analysis of Pyroptosis-Related lncRNAs Signature Associated With Prognosis and Tumor Immune Microenvironment of Pancreatic Adenocarcinoma.与胰腺腺癌预后和肿瘤免疫微环境相关的焦亡相关长链非编码RNA特征的综合分析
Front Genet. 2022 Jul 6;13:899496. doi: 10.3389/fgene.2022.899496. eCollection 2022.
6
Signature of m5C-Related lncRNA for Prognostic Prediction and Immune Responses in Pancreatic Cancer.用于胰腺癌预后预测和免疫反应的m5C相关长链非编码RNA的特征
J Oncol. 2022 Feb 15;2022:7467797. doi: 10.1155/2022/7467797. eCollection 2022.
7
Host-cell interactions in HBV infection and pathogenesis: the emerging role of m6A modification.HBV 感染和发病机制中的宿主细胞相互作用：m6A 修饰的新兴作用。
Emerg Microbes Infect. 2021 Dec;10(1):2264-2275. doi: 10.1080/22221751.2021.2006580.
Hepatology. 2018 Jun;67(6):2254-2270. doi: 10.1002/hep.29683. Epub 2018 Apr 19.
4
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5
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6
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7
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8
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9
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