Rencher Benjamin, Chang Ana M, Fong Hanson, Johnson James D, Paranjpe Avina
Department of Endodontics, School of Dentistry, University of Washington, Seattle, WA, USA.
Department of Periodontics, University of Washington, Seattle, WA, USA.
Restor Dent Endod. 2021 Jun 8;46(3):e35. doi: 10.5395/rde.2021.46.e35. eCollection 2021 Aug.
Endosequence Bioceramic Root Repair Material (BC-RRM) is used in endodontic microsurgery. It is available as a paste and a putty. However, no studies to date have examined the sealing ability of these forms alone or in combination as root-end filling materials. Hence, this study aimed to compare the sealing properties of these 2 forms of BC-RRM.
Forty-two extracted upper anterior teeth were divided into 3 experimental groups, a positive and negative control. After the root canal treatment, the root ends were resected, retroprepared and retrofilled with either putty, paste + putty or mineral trioxide aggregate (MTA). The teeth were mounted in tubes so the apical 3 mm was submerged in Brain Heart Infusion (BHI) broth. The coronal portions of the canals were inoculated with and BHI broth and incubated for 30 days. The broth in the tubes was analyzed for colony forming units to check for leakage of bacteria from the canal. The teeth from the groups were sectioned and analyzed using scanning electron microscopy (SEM). The Kruskal-Wallis test and analysis of variance were used to analyze the data with a significance level < 0.05.
The BC-RRM and MTA groups showed similar sealing ability. The positive control showed leakage in all samples. The SEM imaging showed the presence of bacteria in all experimental groups at the material-tooth interface.
No significant differences were noted in the experimental groups, providing sufficient evidence that any combination could be effectively used during endodontic microsurgery.
牙髓序列生物陶瓷根充修复材料（BC-RRM）用于牙髓显微手术。它有糊剂和油灰状两种形态。然而，迄今为止尚无研究单独或联合检验这些形态作为根尖充填材料的封闭能力。因此，本研究旨在比较这两种形态的BC-RRM的封闭性能。
42颗拔除的上前牙被分为3个试验组、1个阳性对照组和1个阴性对照组。根管治疗后，切除根尖，进行倒预备并分别用BC-RRM油灰状材料、BC-RRM糊剂 + 油灰状材料或三氧化矿物凝聚体（MTA）进行倒充填。将牙齿固定于试管中，使根尖3mm浸入脑心浸液（BHI）肉汤中。根管冠部接种 和BHI肉汤，孵育30天。分析试管中的肉汤的菌落形成单位，以检查细菌是否从根管中渗漏。对试验组的牙齿进行切片，并用扫描电子显微镜（SEM）分析。采用Kruskal-Wallis检验和方差分析对数据进行分析，显著性水平<0.05。
BC-RRM组和MTA组显示出相似的封闭能力。阳性对照组所有样本均出现渗漏。SEM成像显示，所有试验组的材料与牙齿界面均存在细菌。
试验组之间未发现显著差异，这提供了充分的证据表明，在牙髓显微手术中任何一种组合均可有效使用。
