Department of Endodontics, Universidad Central de Venezuela, Caracas, Venezuela.
Instituto de investigaciones Odontológicas Raul Vicentelli, Universidad Central de Venezuela, Caracas, Venezuela.
Eur Endod J. 2022 Oct;7(3):203-209. doi: 10.14744/eej.2022.36036.
To compare, in vitro, the bioceramic materials (MTA and BC RRM-fast set putty) capacity to prevent microleakage of Enterococcus faecalis over time.
An experimental design was made with forty extracted human teeth, coronally cut, and prepared to be placed in a leakage system under sterile conditions. They were randomly divided into two experimental groups: thirty teeth (fifteen for each of retrograde filling material MTA and BC RRM-fast set putty) and a control group: ten teeth (five positive control, five negative control). The 3 mm root-ends were submerged in a brain-heart infusion broth with a red phenol indicator. The coronal access of each sample was inoculated with E. faecalis every seven days to maintain bacterial viability. The lower chamber was evaluated daily for 30 days to observe the turbidity of the culture medium and establish the presence and day of the filtration. Calculation of the colony-forming units (CFU) was performed for each leaked sample. Fisher's Exact Test was used to verify the association between the presence or absence of leakage of the samples by type of bioceramic material used and the Mann-Whitney U test to verify the existence of a difference between the average of CFU by type of bioceramic material used. The significance level used was α=0.05 and a 95% confidence level, as a decision rule for rejecting the null hypothesis.
Of the total samples prepared for each group, leakage was found in 60.0% (9/15) of the MTA group and 40.0% (6/15) of the BC RRM-fast set putty group. All positive controls filtered on the first day of evaluation, while 20% (1/5) of the negative control leaked in the second week. There was no significant difference in leakege between the two groups, nor concerning the bacterial count (P=0.101) and the type of cement used (P=1.000).
BC RRM-fast set putty was comparable to MTA in resisting bacterial microleakage during the observation time.
比较生物陶瓷材料（MTA 和 BC RRM-速凝腻子）在不同时间预防粪肠球菌微渗漏的能力。
对 40 颗人离体牙进行实验设计，牙冠部截断，在无菌条件下置于渗漏系统中进行制备。将样本随机分为两组：30 颗牙（每组各有 15 颗用于逆行充填材料 MTA 和 BC RRM-速凝腻子）和一个对照组：10 颗牙（5 个阳性对照，5 个阴性对照）。将 3mm 根尖端浸入含红色苯酚指示剂的脑心浸液肉汤中。每七天对每个样本的牙冠部进行接种粪肠球菌以保持细菌活力。每天评估下室，观察 30 天，以观察培养基的浊度并确定滤过的存在和日期。对每个泄漏样本进行菌落形成单位（CFU）的计算。Fisher 确切检验用于验证样本泄漏的有无与所用生物陶瓷材料类型之间的关联，Mann-Whitney U 检验用于验证所用生物陶瓷材料类型之间 CFU 平均值是否存在差异。使用的显著性水平为 α=0.05 和 95%置信水平，作为拒绝零假设的决策规则。
每组制备的总样本中，MTA 组有 60.0%（9/15）发生渗漏，BC RRM-速凝腻子组有 40.0%（6/15）发生渗漏。所有阳性对照在评估的第一天就发生了滤过，而 20%（1/5）的阴性对照在第二周发生了渗漏。两组之间的渗漏没有显著差异，也与细菌计数（P=0.101）和使用的水泥类型（P=1.000）无关。
在观察期间，BC RRM-速凝腻子与 MTA 相比，在抵抗细菌微渗漏方面具有可比性。
