肥胖小鼠的血清 25OHD3 受肝损伤影响，并与睾酮水平和精子活力相关。
Serum 25OHD3 of Obese Mice Is Affected by Liver Injury and Correlates with Testosterone Levels and Sperm Motility.
机构信息
College of Life Sciences, Zhaoqing University, Zhaoqing, China.
Guangdong Provincial Key Laboratory of Agro-animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, China.
出版信息
Obes Facts. 2021;14(5):559-567. doi: 10.1159/000518199. Epub 2021 Aug 20.
INTRODUCTION
The concentration of 25-hydroxycholecalciferol (25OHD3) in the serum of obese people is low and often accompanied by symptoms of low fertility. Therefore, vitamin D is recommended as a potential treatment option. However, after clinical trials, it was found that vitamin D cannot effectively increase the concentration of 25OHD3 in the serum of obese people. How obesity causes low 25OHD3 concentration and low fertility is unclear.
METHODS
We analyzed the physiological and pathological changes in obese mice induced by a high-fat diet (HFD) and the changes in mice after supplementing with 25OHD3.
RESULTS
The concentration of 25OHD3 in the serum of obese mice induced by HFD was significantly reduced, and these mice showed liver hypertrophy accompanied by abnormal liver injury, testicular hypertrophy, low testosterone levels, high leptin levels, and low sperm motility. The mRNA and protein expression of CYP2R1 of hydroxylated vitamin D3 was significantly reduced; CYP11A1 and CYP11A2, which synthesize testosterone, were significantly reduced. After supplementing with 25OHD3, there was an increase in serum 25OHD3 concentration, testosterone level, and sperm motility, but it cannot improve the degree of obesity, CYP2R1 expression, and liver damage.
CONCLUSION
Our research shows that there is a metabolic interference mediated by 25OHD3 and testosterone between obesity and low sperm motility. The results of this study can provide a scientific basis for studying the mechanism of 25OHD3 and hormone regulation and treating obese people with low sperm motility.
简介
肥胖人群血清 25-羟胆钙化醇（25OHD3）浓度较低，常伴有生育力低下的症状。因此，维生素 D 被推荐为一种潜在的治疗选择。然而，临床试验后发现，维生素 D 不能有效增加肥胖人群血清 25OHD3 浓度。肥胖如何导致 25OHD3 浓度降低和生育力低下尚不清楚。
方法
我们分析了高脂肪饮食（HFD）诱导肥胖小鼠的生理和病理变化，以及补充 25OHD3 后小鼠的变化。
结果
HFD 诱导肥胖小鼠血清 25OHD3 浓度明显降低，这些小鼠表现出肝脏肥大伴有异常肝损伤、睾丸肥大、睾酮水平降低、瘦素水平升高和精子运动能力降低。羟化维生素 D3 的 CYP2R1 的 mRNA 和蛋白表达明显降低；合成睾酮的 CYP11A1 和 CYP11A2 明显降低。补充 25OHD3 后，血清 25OHD3 浓度、睾酮水平和精子运动能力增加，但不能改善肥胖程度、CYP2R1 表达和肝损伤。
结论
我们的研究表明，肥胖和精子运动能力低下之间存在 25OHD3 和睾酮介导的代谢干扰。本研究结果可为研究 25OHD3 和激素调节机制以及治疗肥胖伴精子运动能力低下提供科学依据。
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