Institut für Mikrobiologie, Veterinärmedizinische Universität, A-1210 Wien, Austria.
Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
Int J Syst Evol Microbiol. 2021 Sep;71(9). doi: 10.1099/ijsem.0.004997.
Strain F2A, isolated from the cricket , was subjected to a polyphasic taxonomic characterization. Cells of the strain were rod-shaped, Gram-stain-negative and catalase- and oxidase-positive. It did not assimilate any carbohydrates. The strain's 16S rRNA gene sequence showed highest similarity to QZS01 (96.4 %). The next highest similarity values were found to representatives of related genera (<93 %). The genome size of strain F2A was 3.2 Mbp and the G+C content was 36.4 mol%. Average nucleotide identity values based on blast and MUMmer and average amino acid identity values between strain F2A and QZS01 were 74.29/74.43, 83.88 and 74.70 %, respectively. The quinone system predominantly contained ubiquinone Q-8. In the polar lipid profile, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified phospholipid were detected. The polyamine pattern consisted of the major compounds putrescine and spermidine. Major fatty acids were C ω7 and C and the hydroxyl acids were C 3-OH, C 2-OH and C 3-OH. The diagnostic diamino acid of the peptidoglycan was -diaminopimelic acid. Due to its association with the only species of the genus but its distinctness from we here propose the novel species sp. nov. F2A (=CCM 9136=LMG 32211).
从蟋蟀中分离出的菌株 F2A 进行了多相分类学鉴定。该菌株的细胞呈杆状,革兰氏阴性,过氧化氢酶和氧化酶阳性。它不能同化任何碳水化合物。该菌株的 16S rRNA 基因序列与 QZS01 (96.4%)最相似。与相关属的代表种的相似性次之(<93%)。菌株 F2A 的基因组大小为 3.2 Mbp,GC 含量为 36.4 mol%。基于 blast 和 MUMmer 的平均核苷酸同一性值和菌株 F2A 与 QZS01 之间的平均氨基酸同一性值分别为 74.29/74.43、83.88 和 74.70%。醌系统主要包含泛醌 Q-8。在极性脂类图谱中,检测到二磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油和一种未鉴定的磷脂。聚胺模式由主要化合物腐胺和亚精胺组成。主要脂肪酸为 Cω7 和 C,羟基酸为 C3-OH、C2-OH 和 C3-OH。肽聚糖的诊断二氨基酸是 -二氨基庚二酸。由于它与属中唯一的种有关,但与 明显不同,我们在这里提议新种 。sp. nov. F2A(=CCM 9136=LMG 32211)。