Kämpfer Peter, Busse Hans-Jürgen, McInroy John A, Criscuolo Alexis, Clermont Dominique, Glaeser Stefanie P
1Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
2Institut für Mikrobiologie, Veterinärmedizinische Universität Wien, A-1210 Wien, Austria.
Int J Syst Evol Microbiol. 2019 Jul;69(7):2070-2075. doi: 10.1099/ijsem.0.003434. Epub 2019 May 17.
Two slightly beige-pigmented, Gram-stain-negative, rod-shaped bacterial strains, IMT-291 and IMT-297, were isolated from soil in a field located in Malvern, Alabama, USA. The source soil had been amended with humic acid and continuously used for the cultivation of worms used for fish bait. It is still conceivable that the source of the strains is from the humic acid amendment, although all attempts to isolate the novel phenotypes from the humic acid source have failed. The two strains were identical based on morphology, growth rate and subsequently by 16S rRNA gene sequences, but showed differences in genomic fingerprint patterns generated by rep-PCR. Phylogenetic analysis based on the 16S rRNA gene revealed a placement of the strain in a distinct cluster with Xinfangfangia soli (97.2 % 16S rRNA gene sequence similarity) and in close proximity to the genus Falsirhodobacter with highest 16S rRNA gene sequence similarity of 95.3 % to the type strain of Falsirhodobacter deserti. Sequence similarities to all other type strains were below 95.0 %. The chemotaxonomic analysis showed a clear similarity to the genus Xinfangfangia. The main cellular fatty acids of the strain were C18 : 1 ω7c, 11-methly-C18 : 1 ω7c and C16 : 0. The major quinone was ubiquinone Q-10. Phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol and phosphatidylcholine were predominant in the polar lipid profile. The polyamine pattern contained the major compound spermidine and moderate amounts of putrescine and cadaverine. The diamino acid of the peptidoglycan was meso-diaminopimelic acid. Based on phylogenetic, chemotaxonomic and phenotypic analyses we propose a new species of the genus Xinfangfangia, with the name Xinfangfangiahumi sp. nov. and strain IMT-291 (=LMG 30636=CIP 111625=CCM 8858) as type strain.
从美国阿拉巴马州莫尔文市一块农田的土壤中分离出两株略带米色色素、革兰氏染色阴性、杆状的细菌菌株，IMT - 291和IMT - 297。该源土壤已用腐殖酸改良，并一直用于养殖用作鱼饵的蚯蚓。尽管从腐殖酸源中分离出这些新表型的所有尝试均告失败，但仍可推测这些菌株的来源是腐殖酸改良剂。基于形态、生长速率以及随后的16S rRNA基因序列分析，这两株菌株是相同的，但在rep - PCR产生的基因组指纹图谱上显示出差异。基于16S rRNA基因的系统发育分析表明，该菌株位于一个与土壤新方菌属（16S rRNA基因序列相似性为97.2%）不同的簇中，并且与假红杆菌属关系密切，与沙漠假红杆菌模式菌株的16S rRNA基因序列相似性最高，为95.3%。与所有其他模式菌株的序列相似性均低于95.0%。化学分类分析表明该菌株与新方菌属有明显相似性。该菌株的主要细胞脂肪酸为C18 : 1 ω7c、11 - 甲基 - C18 : 1 ω7c和C16 : 0。主要醌类为泛醌Q - 10。磷脂酰乙醇胺、磷脂酰单甲基乙醇胺、磷脂酰甘油和磷脂酰胆碱在极性脂质谱中占主导地位。多胺模式包含主要化合物亚精胺以及适量的腐胺和尸胺。肽聚糖的二氨基酸为内消旋二氨基庚二酸。基于系统发育、化学分类和表型分析，我们提议将新方菌属中的一个新物种命名为腐殖新方菌（Xinfangfangiahumi sp. nov.），菌株IMT - 291（=LMG 30636=CIP 111625=CCM 8858）为模式菌株。
