Institute for Integrative Systems Biology I2SysBio, Universitat de València-CSIC, Calle del Catedràtic Agustin Escardino Benlloch 9, 46980 Paterna, Spain.
Darwin Bioprospecting Excellence SL. Parc Científic Universitat de València, Calle del Catedràtic Agustin Escardino Benlloch 9, 46980 Paterna, Spain.
Int J Syst Evol Microbiol. 2022 Mar;72(3). doi: 10.1099/ijsem.0.005240.
A novel Gram-stain-negative, non-motile, halophilic bacterium designated strain M10.9X was isolated from the inner sediment of an aluminium can collected from the Mediterranean Sea (València, Spain). Cells of strain M10.9X were rod-shaped and occasionally formed aggregates. The strain was oxidase-negative and catalase-positive, and showed a slightly psychrophilic, neutrophilic and slightly halophilic metabolism. The phylogenetic analyses revealed that strain M10.9X was closely related to E-37 and F028-2. The genomic G+C content of strain M10.9X was 65.2 mol%. The average nucleotide identity and digital DNA-DNA hybridization values were 76.6 and 20.9 %, respectively, confirming its adscription to a new species within the genus . The major cellular fatty acids were C ω7/C ω6 and C. The polar lipids consisted of phosphatidylglycerol, phosphatidylethanolamine, an unidentified aminolipid, an unidentified glycolipid, an unidentified phospholipid and an unidentified lipid. According to the resuts of a polyphasic study, strain M10.9X represents a novel species of the genus for which the name sp. nov. (type strain M10.9X=DSM 112301=CECT 30307) is proposed.
一株新型革兰氏阴性、非运动性、嗜盐菌，命名为 M10.9X 菌株，从地中海（西班牙巴伦西亚）收集的铝罐内部沉积物中分离得到。M10.9X 菌株的细胞呈杆状，偶尔形成聚集体。该菌株氧化酶阴性，过氧化氢酶阳性，表现出微嗜冷、嗜中性和微嗜盐代谢。系统发育分析表明，M10.9X 菌株与 E-37 和 F028-2 密切相关。M10.9X 菌株的基因组 G+C 含量为 65.2mol%。平均核苷酸同一性和数字 DNA-DNA 杂交值分别为 76.6%和 20.9%，证实其属于属内的一个新种。主要细胞脂肪酸为 C ω7/C ω6 和 C。极性脂由磷脂酰甘油、磷脂酰乙醇胺、一种未鉴定的胺脂、一种未鉴定的糖脂、一种未鉴定的磷脂和一种未鉴定的脂组成。根据多相研究的结果，M10.9X 菌株代表了属的一个新型物种，建议将其命名为. nov.（模式菌株 M10.9X=DSM 112301=CECT 30307）。
