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sp. nov.,分离自海洋废弃物。

sp. nov., isolated from marine waste.

机构信息

Institute for Integrative Systems Biology I2SysBio, Universitat de València-CSIC, Calle del Catedràtic Agustin Escardino Benlloch 9, 46980 Paterna, Spain.

Darwin Bioprospecting Excellence SL. Parc Científic Universitat de València, Calle del Catedràtic Agustin Escardino Benlloch 9, 46980 Paterna, Spain.

出版信息

Int J Syst Evol Microbiol. 2022 Mar;72(3). doi: 10.1099/ijsem.0.005240.

Abstract

A novel Gram-stain-negative, non-motile, halophilic bacterium designated strain M10.9X was isolated from the inner sediment of an aluminium can collected from the Mediterranean Sea (València, Spain). Cells of strain M10.9X were rod-shaped and occasionally formed aggregates. The strain was oxidase-negative and catalase-positive, and showed a slightly psychrophilic, neutrophilic and slightly halophilic metabolism. The phylogenetic analyses revealed that strain M10.9X was closely related to E-37 and F028-2. The genomic G+C content of strain M10.9X was 65.2 mol%. The average nucleotide identity and digital DNA-DNA hybridization values were 76.6 and 20.9 %, respectively, confirming its adscription to a new species within the genus . The major cellular fatty acids were C ω7/C ω6 and C. The polar lipids consisted of phosphatidylglycerol, phosphatidylethanolamine, an unidentified aminolipid, an unidentified glycolipid, an unidentified phospholipid and an unidentified lipid. According to the resuts of a polyphasic study, strain M10.9X represents a novel species of the genus for which the name sp. nov. (type strain M10.9X=DSM 112301=CECT 30307) is proposed.

摘要

一株新型革兰氏阴性、非运动性、嗜盐菌,命名为 M10.9X 菌株,从地中海(西班牙巴伦西亚)收集的铝罐内部沉积物中分离得到。M10.9X 菌株的细胞呈杆状,偶尔形成聚集体。该菌株氧化酶阴性,过氧化氢酶阳性,表现出微嗜冷、嗜中性和微嗜盐代谢。系统发育分析表明,M10.9X 菌株与 E-37 和 F028-2 密切相关。M10.9X 菌株的基因组 G+C 含量为 65.2mol%。平均核苷酸同一性和数字 DNA-DNA 杂交值分别为 76.6%和 20.9%,证实其属于属内的一个新种。主要细胞脂肪酸为 C ω7/C ω6 和 C。极性脂由磷脂酰甘油、磷脂酰乙醇胺、一种未鉴定的胺脂、一种未鉴定的糖脂、一种未鉴定的磷脂和一种未鉴定的脂组成。根据多相研究的结果,M10.9X 菌株代表了属的一个新型物种,建议将其命名为. nov.(模式菌株 M10.9X=DSM 112301=CECT 30307)。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f972/9558578/88888d67fa42/ijsem-72-5240-g001.jpg

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