Department of Rehabilitation Medicine, West China Hospital, Sichuan University, Chengdu, China.
Key Laboratory of Rehabilitation Medicine, West China Hospital, Sichuan University, Chengdu, China.
Bioelectromagnetics. 2021 Dec;42(8):616-628. doi: 10.1002/bem.22372. Epub 2021 Sep 13.
Pulsed electromagnetic field (PEMF) therapy and melatonin (MEL) supplementation are expected to be important strategies for the treatment of osteoporosis. The aim of the current study was to investigate the efficacy of PEMF therapy, MEL supplementation, a combination of PEMF therapy, and MEL supplementation (PEMF + MEL) in mice with bilateral ovariectomy (OVX)-induced osteoporosis. Forty 12-week-old female C57/BL mice were randomly assigned to five groups (n = 8/group): OVX, PEMF, MEL, PEMF + MEL, and sham-operation (sham) groups. All mice in the first four groups were subjected to OVX. The mice in the PEMF and PEMF + MEL groups were exposed to PEMF (75 Hz, 1.6 mT, 1 h/day for 12 weeks), while those in the MEL and PEMF + MEL groups were administered MEL (50 mg/kg, i.p.). Body mass, micro-computed tomography, histology, immunohistochemistry, and real-time polymerase chain reaction were performed. PEMF + MEL treatment enhanced bone volume fraction (BV/TV) 2.2-fold over OVX control (P < 0.001) and increased expression levels of collagen type I (COL1) 1.9-fold and bone morphogenetic protein 2 (BMP2) 2.5-fold. PEMF + MEL also reduced the ratio of bone surface/bone volume (BS/BV) by 40% (P < 0.05) and appeared to reduce the number of osteoclasts in the metaphysis area. Preservation of bone value and bone microarchitecture in the combined therapy group were found to be superior to those in the single treatment groups. However, there were no apparent differences between the PEMF and MEL groups. The use of a combination of PEMF therapy and MEL supplementation may be an effective method to treat osteoporosis. © 2021 Bioelectromagnetics Society.
脉冲电磁场（PEMF）治疗和褪黑素（MEL）补充有望成为治疗骨质疏松症的重要策略。本研究旨在探讨 PEMF 治疗、MEL 补充、PEMF 联合 MEL 治疗（PEMF+MEL）对双侧卵巢切除（OVX）诱导骨质疏松症小鼠的疗效。将 40 只 12 周龄雌性 C57/BL 小鼠随机分为 5 组（每组 8 只）：OVX、PEMF、MEL、PEMF+MEL 和假手术（sham）组。前四组所有小鼠均行 OVX。PEMF 和 PEMF+MEL 组小鼠接受 PEMF（75Hz，1.6mT，每天 1 小时，共 12 周）照射，MEL 和 PEMF+MEL 组小鼠腹腔注射 MEL（50mg/kg）。进行体质量、微计算机断层扫描、组织学、免疫组织化学和实时聚合酶链反应检测。与 OVX 对照组相比，PEMF+MEL 治疗使骨体积分数（BV/TV）增加了 2.2 倍（P<0.001），使Ⅰ型胶原（COL1）的表达水平增加了 1.9 倍，骨形态发生蛋白 2（BMP2）的表达水平增加了 2.5 倍。PEMF+MEL 还使骨表面/骨体积比（BS/BV）降低了 40%（P<0.05），并似乎减少了骺区破骨细胞的数量。与单一治疗组相比，联合治疗组的骨值和骨微结构的保存明显更好。然而，PEMF 组和 MEL 组之间没有明显差异。使用 PEMF 治疗联合 MEL 补充可能是治疗骨质疏松症的一种有效方法。
