含氨基酸侧基的聚合物树脂对胆红素的吸附作用。III. 白蛋白存在下的吸附和解吸

Polymer resins with amino acid containing pendants for sorption of bilirubin. III. Adsorption and desorption in the presence of albumin.

作者信息

Henning D S, Clas S D, Brown G R, St-Pierre L E

机构信息

Department of Chemistry, McGill University, Montreal, Canada.

出版信息

Biomater Artif Cells Artif Organs. 1987;15(4):677-86.

PMID:3452425

Abstract

The adsorption of bilirubin from aqueous phosphate buffer solutions (pH = 7.8) containing bovine serum albumin (BSA) by polyacrylamide resins, with pendants consisting of the oligopeptides (Arg)5(Ala)3-, (Arg)2(Ala)3-, (Lys)5(Ala)3-, His(Arg)2(Ala)3-, has been studied at 0 degrees C under N2. The ability of the oligopeptide-resins to retain adsorbed bilirubin in the presence of BSA follows the order (Arg)5(Ala)3- greater than (Arg)2(Ala)3- greater than (Lys)5(Ala)3- = His(Arg)2(Ala)3-resin. The (Arg)5(Ala)3-resin is able to abstract the second bilirubin on the albumin assuming that BSA binds two bilirubin molecules strongly.

摘要

在氮气氛围下于0℃研究了含牛血清白蛋白（BSA）的磷酸盐水溶液（pH = 7.8）中胆红素被聚丙烯酰胺树脂吸附的情况，该树脂带有由寡肽（Arg）5（Ala）3-、（Arg）2（Ala）3-、（Lys）5（Ala）3-、His（Arg）2（Ala）3-组成的侧链。在BSA存在下，寡肽树脂保留吸附胆红素的能力顺序为：（Arg）5（Ala）3-树脂＞（Arg）2（Ala）3-树脂＞（Lys）5（Ala）3-树脂 = His（Arg）2（Ala）3-树脂。假设BSA强烈结合两个胆红素分子，（Arg）5（Ala）3-树脂能够从白蛋白上夺取第二个胆红素。