Food Science and Technology Department, Chung-Ang University, Republic of Korea.
Food Science and Technology Department, Oregon State University, Corvallis, OR 97331, USA.
Mar Pollut Bull. 2021 Nov;172:112927. doi: 10.1016/j.marpolbul.2021.112927. Epub 2021 Sep 8.
Gastroenteritis infections in humans are mainly associated with consumption of Vibrio parahaemolyticus contaminated shellfish, which causes health and economic loss. Virulence factor production, antibiotic resistance profile, and biofilm-forming capacity of Vibrio parahaemolyticus isolates on food and food contact surfaces at 30 °C were investigated to evaluate the antibiotic sensitivity and pathogenic level. Strains of V. parahaemolyticus were isolated from shellfish (e.g., Crassostrea gigas, Venerupis philippinarum, Mytilus coruscus, Anadara kagoshimensis) in Korea. When examined for 17 virulence factor-encoding genes, 53.3, 73.1, 87.1, 87.9, and 90.9% of the isolates were positive for genes encoding TDH, T6SS, T3SS1, T3SS2, and Type I pilus, respectively. All isolates showed resistance to vancomycin, tetracyclines, penicillin, nalidixic acid, and doxycycline, among 26 antibiotics tested, with most isolates resistant to kanamycin (93.5%), ampicillin (96.8%), clindamycin (96.8%), tobramycin (88.7%), amikacin (83.97%), and minocycline (80.7%). Biofilm formation, cell-cell attachment, and motility were high in most isolates. These findings may assist in monitoring the epidemics of the pathogen. Continuous monitoring could help to decrease V. parahaemolyticus infections and improve seafood safety.
人类肠胃炎感染主要与食用受创伤弧菌污染的贝类有关，这会导致健康和经济损失。为了评估抗生素敏感性和致病性水平，我们研究了 30°C 下食品和食品接触面分离的创伤弧菌菌株的毒力因子产生、抗生素耐药谱和生物膜形成能力。创伤弧菌菌株从韩国的贝类（如，中国蛤蜊、菲律宾帘蛤、皱纹盘鲍、栉孔扇贝）中分离出来。在检测到的 17 种毒力因子编码基因中，分别有 53.3%、73.1%、87.1%、87.9%和 90.9%的分离株携带编码耐热直接溶血素（TDH）、T6SS、T3SS1、T3SS2 和 I 型菌毛的基因。在 26 种抗生素中，所有分离株均对万古霉素、四环素类、青霉素、萘啶酸和强力霉素耐药，其中大多数分离株对卡那霉素（93.5%）、氨苄西林（96.8%）、克林霉素（96.8%）、妥布霉素（88.7%）、阿米卡星（83.97%）和米诺环素（80.7%）耐药。大多数分离株的生物膜形成、细胞间附着和运动能力较高。这些发现可能有助于监测病原体的流行情况。持续监测有助于减少创伤弧菌感染，提高海鲜安全性。
